ASH1 DNA-binding transcription repressor ASH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ASH1
Official Full Name: DNA-binding transcription repressor ASH1
Primary source: SGD:S000001668
Locus tag: YKL185W
See related: AllianceGenome:SGD:S000001668; FungiDB:YKL185W; VEuPathDB:YKL185W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of mating type switching; negative regulation of transcription by RNA polymerase II; and positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter. Located in cellular bud and nucleus. Part of Rpd3L complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ASH1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (94499..96265)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ash1 and Tup1 dependent repression of the Saccharomyces cerevisiae HO promoter requires activator-dependent nucleosome eviction.
Title: Ash1 and Tup1 dependent repression of the Saccharomyces cerevisiae HO promoter requires activator-dependent nucleosome eviction.
FACT and Ash1 promote long-range and bidirectional nucleosome eviction at the HO promoter.
Title: FACT and Ash1 promote long-range and bidirectional nucleosome eviction at the HO promoter.
Three X-ray structures of the ASH1-E3 localization element alone, in complex with its nuclear RBP She2p, and in the cytoplasmic synergistic ternary complex with She2p and She3p.
Title: Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.
These results suggest that after localization to the bud tip, a portion of the localized ASH1 mRNA becomes translationally inactive because of binding of Dhh1 and Puf6 to the 5'- and 3'-UTRs of ASH1 mRNA.
Title: Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of ASH1 mRNA represses localized translation of ASH1 in yeast cells.
ASH1 messenger ribonucleoprotein-core factors form stable complexes in absence of cargo RNA at physiological conditions.
Title: ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.
Recent advances have helped to draw an increasingly clear picture on the molecular mechanisms governing ASH1 mRNA localization from its co-transcriptional birth to its delivery at the site of destination
Title: Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.
ASH1 mRNA is transported to the bud tip by the class V myosin Myo4.
Title: Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.
We assessed ASH1 mRNA and Myo4p distribution more directly and found that their localization is impaired in cells expressing globular tail-lacking Myo4p.
Title: The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.
Regulation of CLN3 expression by Ace2 and Ash1 can account for the differential regulation of Start in response to cell size in mothers and daughters.
Title: Daughter-specific transcription factors regulate cell size control in budding yeast.
The present study reveals that the current accepted model for ASH1 mRNA localization does not fully account for the function of She3p in ASH1 mRNA localization.
Title: She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of mating type switching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Rpd3L complex	HDA more info	PubMed
part_of Rpd3L complex	IDA Inferred from Direct Assay more info	PubMed
part_of Rpd3L-Expanded complex	HDA more info	PubMed
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding transcription repressor ASH1

NP_012736.1

Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

94499..96265

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179751.1 → NP_012736.1 TPA: DNA-binding transcription repressor ASH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012736.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX15, P34233

UniProtKB/TrEMBL

A6ZZE0, B3LQS0, C7GJ24, G2WHK7, N1P888

Conserved Domains (1) summary

smart00401
Location:494 → 548

ZnF_GATA; zinc finger binding to DNA consensus sequence [AT]GATA[AG]