inaD inactivation no afterpotential D [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: inaDprovided by FlyBase
Official Full Name: inactivation no afterpotential Dprovided by FlyBase
Primary source: FLYBASE:FBgn0001263
Locus tag: Dmel_CG3504
See related: AllianceGenome:FB:FBgn0001263
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3504; Dmel\CG3504; Ina-D; InaD; INAD
Summary: Enables myosin III binding activity; photoreceptor activity; and signaling receptor complex adaptor activity. Involved in several processes, including detection of light stimulus; negative regulation of opsin-mediated signaling pathway; and sensory perception of sound. Located in rhabdomere. Part of inaD signaling complex. Is expressed in adult head; photoreceptor; photoreceptor neurons; retina; and rhabdomere. Used to study sensorineural hearing loss. Orthologous to human LNX1 (ligand of numb-protein X 1) and LNX2 (ligand of numb-protein X 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 59B3-59B3; 2-101 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (22855688..22858794, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (18743211..18746317, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Guided by the structure of the INAD-NORPA complex, we discover that INADL is probably a mammalian counterpart of INAD. The INADL PDZ89 tandem specifically binds to PLCb4 with a mode that is strikingly similar to that of the INAD-NORPA complex, as revealed by the structure of the INADL PDZ89-PLCb4 CC-PBM complex.
Title: An unexpected INAD PDZ tandem-mediated plcβ binding in Drosophila photo receptors.
show that INAD PDZ3 binds to the TRP channel tail with a new mechanism. A 15-residue C-terminal tail of TRP forms a b hairpin structure and binds to a surface of INAD PDZ3 outside the canonical target-binding aB/bB groove. Amino acid residues from both strands of the TRP b hairpin are engaged in binding, and thus the INAD PDZ3/TRP interaction is highly specific and with high affinity.
Title: An Exquisitely Specific PDZ/Target Recognition Revealed by the Structure of INAD PDZ3 in Complex with TRP Channel Tail.
Through biochemical and structural studies, study shows that the redox potential of PDZ5 is allosterically regulated by its interaction with another INAD domain, PDZ4.
Title: The INAD scaffold is a dynamic, redox-regulated modulator of signaling in the Drosophila eye.
genetic screen of the third chromosome for mutations that result in mislocalization of an INAD-GFP fusion protein
Title: A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells.
TRP associates with INAD via its last 14 residues to facilitate its regulation by eye-PKC that fine-tunes the visual signaling
Title: Anchoring TRP to the INAD macromolecular complex requires the last 14 residues in its carboxyl terminus.
study demonstrates conformational switch mechanism for PDZ domain function & suggest INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction through cycles of conformational change
Title: Dynamic scaffolding in a G protein-coupled signaling system.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calmodulin binding	TAS Traceable Author Statement more info	PubMed
enables myosin III binding	IPI Inferred from Physical Interaction more info	PubMed
enables myosin binding	TAS Traceable Author Statement more info	PubMed
enables photoreceptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor complex adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor complex adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables signaling receptor complex adaptor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to light stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of light stimulus involved in sensory perception	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of opsin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phototransduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phototransduction	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein localization	TAS Traceable Author Statement more info	PubMed
involved_in sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of inaD signaling complex	IPI Inferred from Physical Interaction more info	PubMed
part_of inaD signaling complex	TAS Traceable Author Statement more info	PubMed
located_in rhabdomere	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: inactivation no afterpotential D

Names: CG3504-PA; CG3504-PB; INAD; inaD-PA; inaD-PB; inactivation no after-potential D; inactivation, no after-depolarization; inactivation-no after potential D; inactivation-no-afterpotential D; inactivation-no-afterpotential-D

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

22855688..22858794 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_166566.1 → NP_726260.1 inactivation no afterpotential D, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726260.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q24008, Q9NBV3

UniProtKB/TrEMBL

D3DMI7

Related

FBpp0071820, FBtr0071909

Conserved Domains (1) summary

cd00992
Location:15 → 103

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001259541.1 → NP_001246470.1 inactivation no afterpotential D, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246470.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMF9, D3DMI7

Conserved Domains (1) summary

cd00992
Location:27 → 115

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...