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    Top3alpha Topoisomerase 3alpha [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35236, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Top3alphaprovided by FlyBase
    Official Full Name
    Topoisomerase 3alphaprovided by FlyBase
    Primary source
    FLYBASE:FBgn0040268
    Locus tag
    Dmel_CG10123
    See related
    AllianceGenome:FB:FBgn0040268
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG10123; Dmel\CG10123; DmTop3alpha; dTopoIIIa; top3a; Top3a; top3alpha; topo IIIalpha; Topo IIIalpha
    Summary
    Enables DNA topoisomerase activity. Involved in double-strand break repair via homologous recombination; mitochondrial genome maintenance; and tissue regeneration. Located in mitochondrial nucleoid and nucleus. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Top3alpha in Genome Data Viewer
    Location:
    37E4-37E4; 2-54 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (19498168..19502268)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (19498168..19502268)

    Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Cytosolic thiouridylase subunit 2 Neighboring gene second mitotic wave missing Neighboring gene cysts Neighboring gene Hig-anchoring scaffold protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution. Chen SH, et al. J Biol Chem, 2012 Jun 1. PMID 22511792, Free PMC Article
    2. Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells. Wu J, et al. Proc Natl Acad Sci U S A, 2010 Apr 6. PMID 20308575, Free PMC Article
    3. Bloom syndrome helicase in meiosis: Pro-crossover functions of an anti-crossover protein. Hatkevich T, et al. Bioessays, 2017 Sep. PMID 28792069, Free PMC Article
    4. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Jiang YF, et al. Sci Rep, 2017 Mar 30. PMID 28358017, Free PMC Article
    5. Cloning and characterization of Drosophila topoisomerase IIIbeta. Relaxation of hypernegatively supercoiled DNA. Wilson TM, et al. J Biol Chem, 2000 Jan 21. PMID 10636841

    See all (47) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The results reported here demonstrate that mitochondrial Top3alpha ablation results in mitochondrial genome instability and its dysfunction, thereby accelerating the aging process.
      Title: Drosophila mitochondrial topoisomerase III alpha affects the aging process via maintenance of mitochondrial function and genome integrity.
    2. the Top3alpha C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA) and an essential function in vivo.
      Title: Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution.
    3. described the phenotypes associated with mitochondrial dysfunction, including the female sterility and the loss of germ-line stem cells of males, as a result from topo IIIalpha deprivation.
      Title: Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells.
    4. partially relaxes a hypernegatively supercoiled plasmid substrate consistent with other purified topo IIIs.
      Title: Drosophila melanogaster topoisomerase IIIalpha preferentially relaxes a positively or negatively supercoiled bubble substrate and is essential during development.
    5. Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration
      Title: Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in developmental growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair via homologous recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitochondrial genome maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tissue regeneration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of RecQ family helicase-topoisomerase III complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrial nucleoid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    topoisomerase 3alpha
    Names
    CG10123-PA
    Top 3alpha
    Top3alpha-PA
    Topoisomerase3alpha
    topoisomerase 3-alpha
    topoisomerase IIIa
    topoisomerase IIIalpha
    NP_523602.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033779.5 Reference assembly

      Range
      19498168..19502268
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_078878.3 → NP_523602.2  topoisomerase 3alpha [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_523602.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q6AWG6, Q9NG98, Q9VIV1
      Related
      FBpp0080787
      Conserved Domains (6) summary
      cd03362
      Location:27 → 185
      TOPRIM_TopoIA_TopoIII; TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving ...
      COG0550
      Location:27 → 618
      TopA; DNA topoisomerase IA [Replication, recombination and repair]
      COG0551
      Location:658 → 744
      YrdD; ssDNA-binding Zn-finger and Zn-ribbon domains of topoisomerase 1 [Replication, recombination and repair]
      cd00186
      Location:194 → 608
      TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
      pfam01396
      Location:657 → 694
      zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger
      pfam06839
      Location:1150 → 1193
      zf-GRF; GRF zinc finger
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9NG98.2 Q9NG98

    Gene LinkOut

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