piwi P-element induced wimpy testis [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: piwiprovided by FlyBase
Official Full Name: P-element induced wimpy testisprovided by FlyBase
Primary source: FLYBASE:FBgn0004872
Locus tag: Dmel_CG6122
See related: AllianceGenome:FB:FBgn0004872
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG6122; Dmel\CG6122; Piwi; PIWI
Summary: Enables RNA endonuclease activity; chromatin DNA binding activity; and piRNA binding activity. Involved in several processes, including germ cell development; pole cell formation; and regulatory ncRNA-mediated gene silencing. Located in several cellular components, including chromatin; chromocenter; and nucleus. Part of RNA polymerase II transcription repressor complex. Is active in cytoplasmic ribonucleoprotein granule; cytosol; and nucleus. Is expressed in several structures, including adult brain; adult fat body; germline cell; gonad; and somatic cell of testis. Used to study primary ovarian insufficiency. Orthologous to human PIWIL1 (piwi like RNA-mediated gene silencing 1) and PIWIL3 (piwi like RNA-mediated gene silencing 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 32C1-32C1; 2-44 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (10982205..10987420, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (10982205..10987420, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila.
Title: Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila.
Direct interaction of Su(var)2-10 via the SIM-binding site of the Piwi protein is required for transposon silencing in Drosophila melanogaster.
Title: Direct interaction of Su(var)2-10 via the SIM-binding site of the Piwi protein is required for transposon silencing in Drosophila melanogaster.
DNMT3L interacts with Piwi and modulates the expression of piRNAs in transgenic Drosophila.
Title: DNMT3L interacts with Piwi and modulates the expression of piRNAs in transgenic Drosophila.
Piwi expressed in Drosophila adipose tissues regulates systemic IGF signaling and growth via IGF-binding protein.
Title: Piwi expressed in Drosophila adipose tissues regulates systemic IGF signaling and growth via IGF-binding protein.
Interaction effect of mutations in the genes (piwi and aub) of the Argonaute family and hobo transposons on the integral survival parameters of Drosophila melanogaster.
Title: Interaction effect of mutations in the genes (piwi and aub) of the Argonaute family and hobo transposons on the integral survival parameters of Drosophila melanogaster.
Piwi suppresses transcription of Brahma-dependent transposons via Maelstrom in ovarian somatic cells.
Title: Piwi suppresses transcription of Brahma-dependent transposons via Maelstrom in ovarian somatic cells.
Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery.
Title: Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery.
Complex Genetic Interactions between Piwi and HP1a in the Repression of Transposable Elements and Tissue-Specific Genes in the Ovarian Germline.
Title: Complex Genetic Interactions between Piwi and HP1a in the Repression of Transposable Elements and Tissue-Specific Genes in the Ovarian Germline.
Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.
Title: Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.
Diverse Defenses: A Perspective Comparing Dipteran Piwi-piRNA Pathways.
Title: Diverse Defenses: A Perspective Comparing Dipteran Piwi-piRNA Pathways.

Submit: New GeneRIF Correction See all GeneRIFs (63)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c10984863-10984721 mobile_element_type = transposon:Cr1a{}6251

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables chromatin DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in female germ-line stem cell asymmetric division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-line stem cell population maintenance	IDA Inferred from Direct Assay more info	PubMed
involved_in germarium-derived oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male germ-line stem cell asymmetric division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by piRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in P granule	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II transcription repressor complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in Yb body	IDA Inferred from Direct Assay more info	PubMed
is_active_in Yb body	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in Yb body	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromocenter	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in heterochromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: P-element induced wimpy testis

Names: CG6122-PA; CG6122-PB; piwi; piwi-PA; piwi-PB

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

10982205..10987420 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057527.4 → NP_476875.1 P-element induced wimpy testis, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476875.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C0PTU6, K7WKS7, K7WKT2, K7WQ39, K7WS94, K7XHZ2, L0CPR8, L0CPS4, L0CQ04, L0CR36, L0CRH9, L0CRI5, L0CRU0, O96674, O96675, Q6NNZ4, Q6NP34, Q9VKM1

UniProtKB/TrEMBL

A0A075BJ92, X2J7U0

Related

FBpp0079755

Conserved Domains (3) summary

smart00949
Location:262 → 396

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:382 → 826

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...

pfam16486
Location:91 → 201

ArgoN; N-terminal domain of argonaute
NM_001298896.1 → NP_001285825.1 P-element induced wimpy testis, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285825.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C0PTU6, K7WKS7, K7WKT2, K7WQ39, K7WS94, K7XHZ2, L0CPR8, L0CPS4, L0CQ04, L0CR36, L0CRH9, L0CRI5, L0CRU0, O96674, O96675, Q6NNZ4, Q6NP34, Q9VKM1

UniProtKB/TrEMBL

A0A075BJ92, X2J7U0

Conserved Domains (3) summary

smart00949
Location:262 → 396

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:382 → 826

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...

pfam16486
Location:91 → 201

ArgoN; N-terminal domain of argonaute