ncd non-claret disjunctional [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: ncdprovided by FlyBase
Official Full Name: non-claret disjunctionalprovided by FlyBase
Primary source: FLYBASE:FBgn0002924
Locus tag: Dmel_CG7831
See related: AllianceGenome:FB:FBgn0002924
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: ca; ca[nd]; cd; CG7831; Dmel\CG7831; DmNcd; DmNCD; KIF C1; KIFC1; l(3)05884; Ncd; NCD; ndc
Summary: Enables protein homodimerization activity. Involved in several processes, including distributive segregation; microtubule cytoskeleton organization; and regulation of cell cycle process. Located in microtubule cytoskeleton and nucleus. Colocalizes with cortical microtubule cytoskeleton and perinuclear region of cytoplasm. Is expressed in several structures, including antennal primordium; central brain primordium; embryonic central brain neurons; ganglia; and head epidermis primordium. Orthologous to human KIFC1 (kinesin family member C1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 99C1-99C2; 3-99 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (29804290..29807127, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (25630012..25632849, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other.
Title: Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other.
the stable compact conformation of Ncd may represent an initial state of the working stroke.
Title: Interactions between motor domains in kinesin-14 Ncd - a molecular dynamics study.
In vitro experiments using the drosophila kinesin-14, non-claret disjunctional (Ncd) show that the diffusive anchorage indeed impacts the force that kinesin-14 motors can generate between two microtubules and that modifications of the anchorage interfaces may act as a regulatory factor influencing the force generation by kinesin-14.
Title: Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.
14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes.
Title: 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes.
tip-tracking of kinesin-14 occurs through several concurrent mechanisms: binding of kinesin only to EB1 located at the MT end, interaction of the kinesin molecules with a composite site formed by EB1 and the MT tip, and probably surface diffusion of the tail along MT.
Title: Insights into the process of EB1-dependent tip-tracking of kinesin-14 Ncd. The role of the microtubule.
Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.
Title: Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.
Mutation of an invariant residue in loop L7 of the central beta-sheet of the Drosophila kinesin-14 Ncd motor alters both nucleotide and microtubule binding, although the mutated residue is not present in either site.
Title: Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant.
Data suggest that the C-terminal residues of Ncd play an important role in modulating the interaction of the motor with the microtubule.
Title: The C-terminus of kinesin-14 Ncd is a crucial component of the force generating mechanism.
Ensemble averaging for each event category revealed that plus- and minus-directed strokes have different size and occur at different instants within the ncd-microtubules attachment sequence.
Title: Bidirectional power stroke by ncd kinesin.
mutation of a single residue in the kinesin-14 Ncd causes the motor to release ADP and hydrolyze ATP faster than wild type
Title: A kinesin motor in a force-producing conformation.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables minus-end-directed microtubule motor activity	NAS Non-traceable Author Statement more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in distributive segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in distributive segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mRNA transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule bundle formation	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in minus-end directed microtubule sliding	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic centrosome separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic spindle assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly involved in female meiosis	NAS Non-traceable Author Statement more info	PubMed
involved_in spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cortical microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in meiotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: non-claret disjunctional

Names: CG7831-PA; CG7831-PB; Kinesin3A; Non-Claret disjunctional; claret nondisjunctional; kinesin-14; ncd-PA; ncd-PB; non-claret disjunction; non-claret-disjunctional; nonclaret disjunction; nonclaret disjunctional

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

29804290..29807127 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001300663.1 → NP_001287592.1 non-claret disjunctional, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287592.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P20480, Q9VAG8

UniProtKB/TrEMBL

A0A0B4LI25, B6UXE6

Conserved Domains (2) summary

smart00129
Location:348 → 678

KISc; Kinesin motor, catalytic domain. ATPase

cd01366
Location:346 → 672

KISc_C_terminal; Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins
NM_057303.5 → NP_476651.1 non-claret disjunctional, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476651.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P20480, Q9VAG8

UniProtKB/TrEMBL

A0A0B4LI25, B6UXE6

Related

FBpp0084900

Conserved Domains (2) summary

smart00129
Location:348 → 678

KISc; Kinesin motor, catalytic domain. ATPase

cd01366
Location:346 → 672

KISc_C_terminal; Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins