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    prmC protein-(glutamine-N(5)) methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945779, updated on 3-Dec-2024

    Summary

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    Official Symbol
    prmC
    Official Full Name
    protein-(glutamine-N(5)) methyltransferase
    Primary source
    ECOCYC:EG12424
    Locus tag
    b1212
    See related
    ASAP:ABE-0004068
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1200; hemK
    Summary
    prmC(hemK) was originally isolated as a suppressor of the light sensitivity of hemH, now likely to be an indirect effect. [More information is available at EcoGene: EG12424]. PrmC is a protein-(glutamine-N5) methyltransferase that shows activity toward polypeptide chain release factors RF1 and RF2 . [More information is available at EcoCyc: EG12424].
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    Genomic context

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    Sequence:
    NC_000913.3 (1266094..1266927)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase Neighboring gene outer membrane lipoprotein LolB Neighboring gene glutamyl-tRNA reductase Neighboring gene peptide chain release factor RF1 Neighboring gene SirB family protein YchQ Neighboring gene transglutaminase-like/TPR repeat-containing protein Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase. Yang Z, et al. J Mol Biol, 2004 Jul 16. PMID 15223314, Free PMC Article
    2. The methylator meets the terminator. Clarke S. Proc Natl Acad Sci U S A, 2002 Feb 5. PMID 11830650, Free PMC Article
    3. Molecular basis for bacterial class I release factor methylation by PrmC. Graille M, et al. Mol Cell, 2005 Dec 22. PMID 16364916
    4. Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a "missing" zeta subfamily of adenine methyltransferases? A hypothesis. Bujnicki JM, et al. IUBMB Life, 1999 Sep. PMID 10690633
    5. Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo. Mora L, et al. J Biol Chem, 2007 Dec 7. PMID 17932046

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. HemK is a Protein-glutamine N- methyltransferase
      Title: The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
    2. HemK is a Protein-glutamine N- methyltransferase
      Title: HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
    3. Results describe the crystal structure of the complex between E. coli RF1 and PrmC bound to the methyl donor product AdoHCy.
      Title: Molecular basis for bacterial class I release factor methylation by PrmC.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables S-adenosylmethionine-dependent methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables S-adenosylmethionine-dependent methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-(glutamine-N5) methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-glutamine N-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-glutamine N-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-glutamine N-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in peptidyl-glutamine methylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein methylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translational termination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translational termination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    protein-(glutamine-N(5)) methyltransferase
    NP_415730.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1266094..1266927
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415730.1 protein-(glutamine-N(5)) methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415730.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN5FWA8
      Conserved Domains (1) summary
      TIGR00536
      Location:1 → 277
      hemK_fam; HemK family putative methylases
    Nucleotide Protein
    Heading Accession and Version

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