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    rplD 50S ribosomal subunit protein L4 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947818, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rplD
    Official Full Name
    50S ribosomal subunit protein L4
    Primary source
    ECOCYC:EG10867
    Locus tag
    b3319
    See related
    ASAP:ABE-0010858
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3306; eryA
    Summary
    The L4 protein is a component of the 50S subunit of the ribosome and also regulates the expression of S10 operon at both the transcriptional and posttranscriptional level. [More information is available at EcoCyc: EG10867].
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    Genomic context

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    Sequence:
    NC_000913.3 (3451681..3452286, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit protein S19 Neighboring gene 50S ribosomal subunit protein L2 Neighboring gene 50S ribosomal subunit protein L23 Neighboring gene 50S ribosomal subunit protein L3 Neighboring gene 30S ribosomal subunit protein S10

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation. Freedman LP, et al. Proc Natl Acad Sci U S A, 1987 Sep. PMID 2442760, Free PMC Article
    2. A hairpin structure upstream of the terminator hairpin required for ribosomal protein L4-mediated attenuation control of the S10 operon of Escherichia coli. Zengel JM, et al. J Bacteriol, 1996 Apr. PMID 8636042, Free PMC Article
    3. Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions. Li X, et al. RNA, 1996 Jan. PMID 8846294, Free PMC Article
    4. Changes in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation analysis. Papadopoulos G, et al. Eur Biophys J, 2006 Oct. PMID 16773394
    5. Thermus thermophilus L4 ribosomal protein: purification and sensitivity alteration against erythromycin of E. coli cells harboring a single amino acid mutant of TthL4 within its extended loop. Leontiadou F, et al. Amino Acids, 2007 Sep. PMID 17048124

    See all (68) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Posttranscriptional Regulation of tnaA by Protein-RNA Interaction Mediated by Ribosomal Protein L4 in Escherichia coli.
      Title: Posttranscriptional Regulation of tnaA by Protein-RNA Interaction Mediated by Ribosomal Protein L4 in Escherichia coli.
    2. The extended loops of ribosomal proteins L4 and L22 of Escherichia coli contribute to ribosome assembly and protein translation.
      Title: The extended loops of ribosomal proteins uL4 and uL22 of Escherichia coli contribute to ribosome assembly and protein translation.
    3. The effects of classical erythromycin resistance mutations in ribosomal proteins L4 and L22 of the large ribosomal subunit on the kinetics of erythromycin binding, were characterized.
      Title: Erythromycin resistance by L4/L22 mutations and resistance masking by drug efflux pump deficiency.
    4. Our results highlight the important role of L4 and L22 in ribosome function and assembly, and indicate that a variety of changes in these proteins can mediate macrolide resistance.
      Title: Novel mutations in ribosomal proteins L4 and L22 that confer erythromycin resistance in Escherichia coli.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables RNA-binding transcription regulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endoribonuclease inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA 5'-UTR binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables translation repressor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation repressor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA-templated transcription termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of cytoplasmic translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribosome assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within transcriptional attenuation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of large ribosomal subunit IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    50S ribosomal subunit protein L4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3451681..3452286 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417778.1 50S ribosomal subunit protein L4 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417778.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AQ98
      Conserved Domains (1) summary
      PRK05319
      Location:1 → 201
      rplD; 50S ribosomal protein L4; Provisional
    Nucleotide Protein
    Heading Accession and Version

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