greB transcription elongation factor GreB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: greB
Official Full Name: transcription elongation factor GreB
Primary source: ECOCYC:EG11578
Locus tag: b3406
See related: ASAP:ABE-0011117
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3393
Summary: GreB stimulates the mRNA cleavage activity of RNA polymerase , which acts to release a polymerase complex that has stalled . [More information is available at EcoCyc: EG11578].
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Genomic context

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Sequence:: NC_000913.3 (3536812..3537288)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.
Title: Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.
Data suggest that rRNA promoters are inhibited by the secondary channel factors (SCFs) transcription factors GreB and DksA because their short-lived RNA polymerases (RNAPs) complexes do not allow sufficient time for SCFs to dissociate.
Title: Delayed inhibition mechanism for secondary channel factor regulation of ribosomal RNA transcription.
These reconstructions provide insight into the catalytic mechanism and dynamics of RNA cleavage and extension and suggest how GreB targets backtracked complexes without interfering with canonical transcription
Title: Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation.
GreB binds to actively elongating complexes at nearly diffusion-limited rates but remains bound for only 0.3-0.5 s. Bound GreB inhibits transcript elongation only partially. GreB binds rapidly and randomly to elongation complexes, patrolling for those requiring nucleolytic rescue, and its short residence time minimizes RNAP inhibition
Title: Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue.
overexpression of GreB in DeltagreA cells reduced the misincorporation errors to wild-type levels, demonstrating that GreB at high concentration could substitute for GreA in RNA proofreading activity in vivo.
Title: A Cre Transcription Fidelity Reporter Identifies GreA as a Major RNA Proofreading Factor in Escherichia coli.
Results show that although DksA and GreB compete for binding to free RNA polymerase, they do not interfere with each other's activities during elongation.
Title: An insertion in the catalytic trigger loop gates the secondary channel of RNA polymerase.
Data report the structure of Escherichia coli GreB, and show that the carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors.
Title: The carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors.
N-terminus verified by Edman degradation on complete protein
Title: Transcript cleavage factors from E. coli.
We show that the apparent affinities of DksA and GreB for RNAP are similar, but the cellular concentration of GreB is much lower than that of DksA.
Title: Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase.
The half-life of GreA and GreB proteins suggests the involvement of DnaK and DnaJ in the stability of these proteins.
Title: Influence of Escherichia coli DnaK and DnaJ molecular chaperones on tryptophanase (TnaA) amount and GreA, GreB stability.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase binding	IEA Inferred from Electronic Annotation more info
enables transcription elongation factor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription elongation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription elongation	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription elongation factor GreB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3536812..3537288

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

YP_026216.2 transcription elongation factor GreB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for YP_026216.2

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376TRJ1

Conserved Domains (1) summary

PRK01885
Location:1 → 156

greB; transcription elongation factor GreB; Reviewed