zuc zucchini [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: zucprovided by FlyBase
Official Full Name: zucchiniprovided by FlyBase
Primary source: FLYBASE:FBgn0261266
Locus tag: Dmel_CG12314
See related: AllianceGenome:FB:FBgn0261266
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG12314; Dmel\CG12314; MitoPLD; Zuc; ZUC
Summary: Enables RNA endonuclease activity, producing 5'-phosphomonoesters and cardiolipin hydrolase activity. Involved in oocyte karyosome formation and primary piRNA processing. Located in P granule and mitochondrion. Is active in cytoplasmic side of mitochondrial outer membrane. Is expressed in several structures, including anterior endoderm; anterior endoderm anlage; embryonic/larval midgut; embryonic/larval midgut primordium; and mesoderm. Orthologous to human PLD6 (phospholipase D family member 6). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 33B5-33B5; 2-45 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (11988735..11989882, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (11988735..11989882, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endogenous piRNA-guided slicing triggers responder and trailer piRNA production from viral RNA in Aedes aegypti mosquitoes.
Title: Endogenous piRNA-guided slicing triggers responder and trailer piRNA production from viral RNA in Aedes aegypti mosquitoes.
A heterologous RNA that lacks complementary piRNAs is processed into piRNAs upon recruitment of several piRNA pathway factors. This processing requires Zuc and the helicase Armitage (Armi).
Title: Zucchini-dependent piRNA processing is triggered by recruitment to the cytoplasmic processing machinery.
these data uncover an evolutionarily conserved piRNA biogenesis mechanism in which Zucchini plays a central role in defining piRNA 5' and 3' ends.
Title: Noncoding RNA. piRNA-guided slicing specifies transcripts for Zucchini-dependent, phased piRNA biogenesis.
preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster
Title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster.
The active site is situated on the dimer interface at the bottom of a narrow groove that can likely accommodate single-stranded nucleic acid substrates.
Title: Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.
Zucchini (Zuc), a mitochondrial phospholipase D (PLD) superfamily member, is an endoribonuclease essential for primary piRNA biogenesis; crystal structure of Drosophila melanogaster Zuc (DmZuc) at 1.75 A resolution
Title: Structure and function of Zucchini endoribonuclease in piRNA biogenesis.
Loss of Zucchini leads to an accumulation of Piwi and Armitage in Yb bodies, indicating that Yb bodies are sites of primary piRNA biogenesis.
Title: An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IMP Inferred from Mutant Phenotype more info	PubMed
enables cardiolipin hydrolase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in dorsal appendage formation	HMP more info	PubMed
involved_in oocyte karyosome formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	HMP more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
NOT located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic side of mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: zucchini

Names: CG12314-PA; zuc-PA; zuchini

NP_609530.1

EC 3.1.26.-

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

11988735..11989882 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_135686.3 → NP_609530.1 zucchini [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_609530.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0AP42, A0AP43, A0AP44, A0AP47, A0AP48, A0AP51, Q9VKD7

UniProtKB/TrEMBL

L0CRJ9

Related

FBpp0079900

Conserved Domains (2) summary

pfam13091
Location:96 → 239

PLDc_2; PLD-like domain

cl15239
Location:87 → 239

PLDc_SF; Catalytic domain of phospholipase D superfamily proteins