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    nrfB periplasmic nitrite reductase penta-heme c-type cytochrome [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948573, updated on 17-Dec-2024

    Summary

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    Official Symbol
    nrfB
    Official Full Name
    periplasmic nitrite reductase penta-heme c-type cytochrome
    Primary source
    ECOCYC:EG11945
    Locus tag
    b4071
    See related
    ASAP:ABE-0013344
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4064; yjcI
    Summary
    SignalP strongly predicts a signal of 32 aa (34 aa if a GTG start at -2 is used), as previously noted (Clarke, 2004), but N-terminal sequencing found a cleavage after 25 residues (Clarke, 2007). [More information is available at EcoGene: EG11945]. The nrfB gene encodes a cytochrome C that is believed to be the direct electron donor for cytochrome C552 in the formate-dependent reduction of nitrite to ammonia. [More information is available at EcoCyc: EG11945].
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    Genomic context

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    Sequence:
    NC_000913.3 (4289245..4289811)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YtcB Neighboring gene cytochrome c552 nitrite reductase Neighboring gene putative menaquinol-cytochrome c reductase 4Fe-4S subunit Neighboring gene putative menaquinol-cytochrome c reductase subunit NrfD Neighboring gene putative cytochrome c-type biogenesis protein NrfE Neighboring gene putative formate-dependent nitrite reductase complex subunit NrfF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA. Clarke TA, et al. Biochem J, 2007 Aug 15. PMID 17521287, Free PMC Article
    2. Purification and spectropotentiometric characterization of Escherichia coli NrfB, a decaheme homodimer that transfers electrons to the decaheme periplasmic nitrite reductase complex. Clarke TA, et al. J Biol Chem, 2004 Oct 1. PMID 15280383
    3. Role of a conserved glutamine residue in tuning the catalytic activity of Escherichia coli cytochrome c nitrite reductase. Clarke TA, et al. Biochemistry, 2008 Mar 25. PMID 18311941
    4. The nitric oxide reductase activity of cytochrome c nitrite reductase from Escherichia coli. van Wonderen JH, et al. J Biol Chem, 2008 Apr 11. PMID 18245085
    5. Respiratory detoxification of nitric oxide by the cytochrome c nitrite reductase of Escherichia coli. Poock SR, et al. J Biol Chem, 2002 Jun 28. PMID 11960983

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
      Title: The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables electron transfer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nitrite reductase (cytochrome, ammonia-forming) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables oxidoreductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in anaerobic respiration IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    periplasmic nitrite reductase penta-heme c-type cytochrome

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4289245..4289811
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418495.2 periplasmic nitrite reductase penta-heme c-type cytochrome [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418495.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0H3JMG5, A0A1X3J930, C3SH92
      Conserved Domains (1) summary
      PRK11659
      Location:4 → 186
      PRK11659; cytochrome c nitrite reductase pentaheme subunit; Provisional
    Nucleotide Protein
    Heading Accession and Version

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