U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    mfd transcription-repair coupling factor [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945681, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    mfd
    Official Full Name
    transcription-repair coupling factor
    Primary source
    ECOCYC:EG11619
    Locus tag
    b1114
    See related
    ASAP:ABE-0003763
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1100
    Summary
    Mfd dissociates transcription elongation complexes blocked at nonpairing lesions and mediates recruitment of DNA repair proteins. [More information is available at EcoGene: EG11619]. The Mfd (mutation frequency decline) protein, also known as transcription-repair coupling factor , is responsible for ATP-dependent removal of stalled RNA polymerase from DNA lesions by inducing forward movement of the RNA polymerase and subsequent recruitment of nucleotide excision repair machinery to the sites of the lesions . [More information is available at EcoCyc: EG11619].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (1170518..1173964, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1471 domain-containing multiple stress resistance outer membrane protein BhsA Neighboring gene ncRNA Neighboring gene L,D-transpeptidase LdtC Neighboring gene inner membrane protein YcfT Neighboring gene lipoprotein release complex - inner membrane subunit Neighboring gene lipoprotein release complex - ATP binding subunit

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Structural basis for transcription complex disruption by the Mfd translocase. Kang JY, et al. Elife, 2021 Jan 22. PMID 33480355, Free PMC Article
    2. Transcription-repair coupling determines the strandedness of ultraviolet mutagenesis in Escherichia coli. Oller AR, et al. Proc Natl Acad Sci U S A, 1992 Nov 15. PMID 1438310, Free PMC Article
    3. Structure and function of transcription-repair coupling factor. II. Catalytic properties. Selby CP, et al. J Biol Chem, 1995 Mar 3. PMID 7876262
    4. Mechanisms of transcription-repair coupling and mutation frequency decline. Selby CP, et al. Microbiol Rev, 1994 Sep. PMID 7968917, Free PMC Article
    5. Molecular mechanism of transcription-repair coupling. Selby CP, et al. Science, 1993 Apr 2. PMID 8465200

    See all (47) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural basis for transcription complex disruption by the Mfd translocase.
      Title: Structural basis for transcription complex disruption by the Mfd translocase.
    2. Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA.
      Title: Cotranscriptional R-loop formation by Mfd involves topological partitioning of DNA.
    3. Mfd can locate a stalled/paused transcription elongation complex via a release and catch-up mechanism. Depending on load, Mfd either rescues a backtracked TEC or terminates transcription.
      Title: Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism.
    4. The results reveal that bacterial RNA polymerase is frequently rescued by Mfd during normal growth and highlight a ubiquitous house-keeping role for Mfd in regulating transcription elongation.
      Title: The transcription-repair coupling factor Mfd associates with RNA polymerase in the absence of exogenous damage.
    5. Mfd, which is an ATP-dependent superfamily 2 helicase that binds to RNAP, continues to translocate along DNA after RNAP has been displaced until a lesion in the template strand is located.
      Title: Stalled transcription complexes promote DNA repair at a distance.
    6. TRCF derepression is associated with profound conformational changes that primarily involve a reorganization of the interdomain interactions.
      Title: Derepression of bacterial transcription-repair coupling factor is associated with a profound conformational change.
    7. Mfd might form a excinuclease ABC subunit A recruitment factor at stalled transcription complexes that architecturally
      Title: Structural basis for transcription-coupled repair: the N terminus of Mfd resembles UvrB with degenerate ATPase motifs.
    8. preliminary structure determination of Escherichia coli Mfd, the transcription-repair coupling factor
      Title: Crystallization and preliminary structure determination of Escherichia coli Mfd, the transcription-repair coupling factor.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA translocase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA translocase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase core enzyme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase core enzyme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables damaged DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    NOT enables helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, preincision complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within transcription-coupled nucleotide-excision repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within transcription-coupled nucleotide-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within transcription-coupled nucleotide-excision repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcription-coupled nucleotide-excision repair, DNA damage recognition IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within transcription-coupled nucleotide-excision repair, DNA damage recognition IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transcription-coupled nucleotide-excision repair, DNA damage recognition IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of DNA repair complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    transcription-repair coupling factor

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1170518..1173964 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415632.1 transcription-repair coupling factor [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415632.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EJ55, A0A9P2MJT3
      Conserved Domains (1) summary
      PRK10689
      Location:1 → 1147
      PRK10689; transcription-repair coupling factor; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.