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    insI1 IS30 family transposase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949005, updated on 17-Dec-2024

    Summary

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    Official Symbol
    insI1
    Official Full Name
    IS30 family transposase
    Primary source
    ECOCYC:G6131
    Locus tag
    b0256
    See related
    ASAP:ABE-0000871
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0258; tra8-1
    Summary
    [More information is available at EcoGene: EG60004]. This is the transposase for the insertion sequence IS30. [More information is available at EcoCyc: G6131].
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    Genomic context

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    Sequence:
    NC_000913.3 (270603..271754)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative GTP-binding protein YkfA Neighboring gene putative transcriptional regulator PerR Neighboring gene pseudo Neighboring gene ncRNA Neighboring gene PF00078 domain-containing protein YkfC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Sub-terminal sequences modulating IS30 transposition in vivo and in vitro. Szabó M, et al. J Mol Biol, 2008 Jan 11. PMID 18022196
    2. Terminal inverted repeats of insertion sequence IS30 serve as targets for transposition. Olasz F, et al. J Bacteriol, 1997 Dec. PMID 9393723, Free PMC Article
    3. Mutations in the carboxy-terminal part of IS30 transposase affect the formation and dissolution of (IS30)2 dimer. Olasz F, et al. FEBS Lett, 1997 Aug 25. PMID 9303555
    4. The N-terminal domain of the insertion sequence 30 transposase interacts specifically with the terminal inverted repeats of the element. Stalder R, et al. J Biol Chem, 1990 Mar 5. PMID 2154486
    5. Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Hayashi K, et al. Mol Syst Biol, 2006. PMID 16738553, Free PMC Article

    See all (7) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA insertion or deletion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transposase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transposase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA integration IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA transposition IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA transposition IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transposition IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transposition IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of protein-DNA complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    IS30 family transposase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      270603..271754
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414790.1 IS30 family transposase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414790.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TMU5, A0A8S7K4K1
      Conserved Domains (1) summary
      COG2826
      Location:52 → 383
      Tra8; Transposase and inactivated derivatives, IS30 family [Mobilome: prophages, transposons]
    Nucleotide Protein
    Heading Accession and Version

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