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    mreB dynamic cytoskeletal protein MreB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948588, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mreB
    Official Full Name
    dynamic cytoskeletal protein MreB
    Primary source
    ECOCYC:EG10608
    Locus tag
    b3251
    See related
    ASAP:ABE-0010661
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3239; envB; mon; mreB_1; rodY
    Summary
    Overexpression causes abnormal biofilm architecture, reduces motility and inhibits cell division. [More information is available at EcoGene: EG10608]. The mre genes are responsible for rod shape and mecillinam sensitivity in Escherichia coli . [More information is available at EcoCyc: EG10608].
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    Genomic context

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    Sequence:
    NC_000913.3 (3400044..3401087, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNase G Neighboring gene nucleoside triphosphate pyrophosphatase YhdE Neighboring gene cell shape determining protein MreD Neighboring gene cell shape determining protein MreC Neighboring gene regulator of CsrB and CsrC decay Neighboring gene acrylyl-CoA reductase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB. Jena P, et al. Nanoscale, 2020 Feb 14. PMID 31993609
    2. Filament formation of the Escherichia coli actin-related protein, MreB, in fission yeast. Srinivasan R, et al. Curr Biol, 2007 Feb 6. PMID 17276920
    3. Structure-activity relationship study of the bacterial actin-like protein MreB inhibitors: effects of substitution of benzyl group in S-benzylisothiourea. Iwai N, et al. Biosci Biotechnol Biochem, 2007 Jan. PMID 17213642
    4. A22 disrupts the bacterial actin cytoskeleton by directly binding and inducing a low-affinity state in MreB. Bean GJ, et al. Biochemistry, 2009 Jun 9. PMID 19382805, Free PMC Article
    5. Chromosome segregation in Escherichia coli division: a free energy-driven string model. Fan J, et al. Comput Biol Chem, 2007 Aug. PMID 17631415

    See all (91) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB.
      Title: Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB.
    2. MreBA125V and MreBA174T mutant cells were thinner and thicker than WT cells, and MreBA125V and MreBA174T rotated faster and slower than WT, respectively.
      Title: Relation between rotation of MreB actin and cell width of Escherichia coli.
    3. Studies indicate the function of dynamic cytoskeletal protein MreB to generate an integrated model of rod-shaped wall construction [Review].
      Title: How to Build a Bacterial Cell: MreB as the Foreman of E. coli Construction.
    4. MreB mutants alter the bending properties of MreB filaments in molecular dynamics simulations similar to RodZ binding, and these mutants rescue rod-like shape in the absence of RodZ alone or in combination with wild-type MreB.
      Title: RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape.
    5. The data suggest that RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform E. coli cylinders.
      Title: MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity.
    6. MreB is not recruited to the FtsZ ring in secA mutant cells, contributing to division arrest and cell filamentation.. Thus, when we reroute RodZ, MreB membrane-anchor, by fusing it to a SecA-independent integral membrane protein and overproducing it, MreB localization is restored and the defect in cell division is corrected.
      Title: The bacterial Sec system is required for the organization and function of the MreB cytoskeleton.
    7. Although several other protein modulators are known to target FtsZ, the CbtA-interacting surface we identify represents a novel inhibitory target. Our findings establish CbtA as a dual function toxin that inhibits both cell division and cell elongation via direct and independent interactions with FtsZ and MreB.
      Title: CbtA toxin of Escherichia coli inhibits cell division and cell elongation via direct and independent interactions with FtsZ and MreB.
    8. Results demonstrate that the physical properties of MreB filaments are important for shape control and support a model in which MreB organizes the cell wall growth machinery to produce a chiral cell wall structure and dictate cell diameter
      Title: MreB Orientation Correlates with Cell Diameter in Escherichia coli.
    9. E75, R78 and D82 of FtsZ are key residues for FtsZ self-assembly and FtsZ-MreB interaction.
      Title: [E75, R78 and D82 of Escherichia coli FtsZ are key residues for FtsZ cellular self-assembly and FtsZ-MreB interaction].
    10. Data show that actin homolog MreB rotates because cytoplasmic MreB filaments are coupled to periplasmic cell wall synthesis through the transmembrane protein RodZ, which acts as a transmembrane linker.
      Title: RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis.
    Submit: New GeneRIF Correction See all GeneRIFs (33)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within FtsZ-dependent cytokinesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within FtsZ-dependent cytokinesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cell morphogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of cell division IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of cell shape IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of chromosome segregation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of chromosome segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in polymeric cytoskeletal fiber IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    dynamic cytoskeletal protein MreB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3400044..3401087 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417717.2 dynamic cytoskeletal protein MreB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417717.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AS38
      Conserved Domains (1) summary
      PRK13927
      Location:6 → 345
      PRK13927; rod shape-determining protein MreB; Provisional
    Nucleotide Protein
    Heading Accession and Version

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