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    lpxA acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944849, updated on 3-Dec-2024

    Summary

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    Official Symbol
    lpxA
    Official Full Name
    acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
    Primary source
    ECOCYC:EG10545
    Locus tag
    b0181
    See related
    ASAP:ABE-0000618
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0180
    Summary
    LpxA contains 24 hexapeptide repeats. [More information is available at EcoGene: EG10545]. UDP-N-acetylglucosamine acyltransferase (LpxA) catalyzes the first reaction of lipid A biosynthesis. [More information is available at EcoCyc: EG10545].
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    Genomic context

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    Sequence:
    NC_000913.3 (202560..203348)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase Neighboring gene 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase Neighboring gene lipid A disaccharide synthase Neighboring gene RNase HII Neighboring gene DNA polymerase III subunit alpha

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The active site of Escherichia coli UDP-N-acetylglucosamine acyltransferase. Chemical modification and site-directed mutagenesis. Wyckoff TJ, et al. J Biol Chem, 1999 Sep 17. PMID 10480918
    2. The lipid A biosynthesis mutation lpxA2 of Escherichia coli results in drastic antibiotic supersusceptibility. Vuorio R, et al. Antimicrob Agents Chemother, 1992 Apr. PMID 1503445, Free PMC Article
    3. Acyl-acyl carrier protein specificity of UDP-GlcNAc acyltransferases from gram-negative bacteria: relationship to lipid A structure. Williamson JM, et al. J Bacteriol, 1991 Jun. PMID 1904441, Free PMC Article
    4. A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis. Galloway SM, et al. J Biol Chem, 1990 Apr 15. PMID 2180947
    5. Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine. Anderson MS, et al. J Biol Chem, 1987 Apr 15. PMID 3549716

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Overlay of the LpxA-RJPXD33 structure with E. coli LpxD identified a complementary peptide binding pocket within LpxD and serves as a model for further biochemical characterization of RJPXD33 binding to LpxD.
      Title: Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis.
    2. N-terminus verified by Edman degradation on complete protein
      Title: UDP-N-acetylglucosamine acyltransferase of Escherichia coli. The first step of endotoxin biosynthesis is thermodynamically unfavorable.
    3. show how LpxA selects for 14-carbon R-3-hydroxyacyl chains and reveal two modes of UDP binding
      Title: Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase.
    4. Results suggest that nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis.
      Title: Nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis.
    5. compact beta-turn structure of peptide 920 bound to LpxA may open previously uncharacterized approaches to the rational design of LpxA inhibitors with antibiotic activity
      Title: Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in lipid A biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
    NP_414723.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      202560..203348
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414723.1 acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414723.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AM74, Q0P6M6
      Conserved Domains (1) summary
      PRK05289
      Location:4 → 262
      PRK05289; acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase
    Nucleotide Protein
    Heading Accession and Version

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