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    hypB hydrogenase isoenzymes nickel incorporation protein HypB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947194, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hypB
    Official Full Name
    hydrogenase isoenzymes nickel incorporation protein HypB
    Primary source
    ECOCYC:EG10484
    Locus tag
    b2727
    See related
    ASAP:ABE-0008960
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2722; hydB
    Summary
    hypB mutants are pheotypically suppressed by added Ni. [More information is available at EcoGene: EG10484]. The HypB protein is an accessory protein involved in the maturation of all hydrogenase isoenzymes. [More information is available at EcoCyc: EG10484].
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    Genomic context

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    Sequence:
    NC_000913.3 (2851001..2851873)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene regulator of the transcriptional regulator FhlA Neighboring gene hydrogenase 3 nickel incorporation protein HypA Neighboring gene hydrogenase maturation factor HypC Neighboring gene Fe-(CN)2CO cofactor assembly scaffold protein HypD Neighboring gene carbamoyl dehydratase HypE Neighboring gene DNA-binding transcriptional activator FhlA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB. Leach MR, et al. Biochemistry, 2005 Sep 13. PMID 16142921
    2. GTP hydrolysis by HypB is essential for nickel insertion into hydrogenases of Escherichia coli. Maier T, et al. Eur J Biochem, 1995 May 15. PMID 7601092
    3. Maturation of hydrogenases. Böck A, et al. Adv Microb Physiol, 2006. PMID 17091562
    4. Isolation of genes required for hydrogenase synthesis in Escherichia coli. Chaudhuri A, et al. J Gen Microbiol, 1987 Dec. PMID 3332683
    5. Relationship between the GTPase, metal-binding, and dimerization activities of E. coli HypB. Cai F, et al. J Biol Inorg Chem, 2011 Aug. PMID 21544686

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Metal specificity of the Ni(II) and Zn(II) binding sites of the N-terminal and G-domain of E. coli HypB.
      Title: Metal specificity of the Ni(II) and Zn(II) binding sites of the N-terminal and G-domain of E. coli HypB.
    2. analysis of a cell-free approach for H-cluster synthesis and hydrogenase maturation by using separately expressed and purified HydE, HydF, and HydG
      Title: New insights into [FeFe] hydrogenase activation and maturase function.
    3. Data support model in which HypB-SlyD complex establishes communication between GTP hydrolysis and nickel delivery and provide insight into role of HypB-SlyD complex during [NiFe]-hydrogenase biosynthesis.
      Title: Escherichia coli SlyD, more than a Ni(II) reservoir.
    4. N-terminus verified by Edman degradation on complete protein
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    5. model for the function of HypB during the Ni(II) loading of hydrogenase is proposed
      Title: Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metallochaperone activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables nickel cation binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nickel cation binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nickel cation binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein maturation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein maturation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein maturation by nickel ion transfer IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein-containing complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of GTPase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hydrogenase isoenzymes nickel incorporation protein HypB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2851001..2851873
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417207.1 hydrogenase isoenzymes nickel incorporation protein HypB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417207.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FY24
      Conserved Domains (1) summary
      PRK10463
      Location:1 → 290
      PRK10463; hydrogenase nickel incorporation protein HypB; Provisional
    Nucleotide Protein
    Heading Accession and Version

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