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    valS valine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948785, updated on 3-Dec-2024

    Summary

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    Official Symbol
    valS
    Official Full Name
    valine--tRNA ligase
    Primary source
    ECOCYC:EG11067
    Locus tag
    b4258
    See related
    ASAP:ABE-0013946
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4251; val-act
    Summary
    Valyl-tRNA synthetase (ValRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG11067].
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    Genomic context

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    Sequence:
    NC_000913.3 (4480982..4483837, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative N-acetyltransferase YjgM Neighboring gene inner membrane protein YjgN Neighboring gene DNA polymerase III subunit chi Neighboring gene aminopeptidase A/I

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular cloning and characterization of the gene for Escherichia coli valyl-tRNA synthetase. Skogman SG, et al. Gene, 1984 Oct. PMID 6392024
    2. Valyl-tRNA synthetase from Escherichia coli MALDI-MS identification of the binding sites for L-valine or for noncognate amino acids upon qualitative comparative labeling with reactive amino-acid analogs. Hountondji C, et al. Eur J Biochem, 2000 Aug. PMID 10903513
    3. Misactivated amino acids translocate at similar rates across surface of a tRNA synthetase. Nomanbhoy TK, et al. Proc Natl Acad Sci U S A, 2000 May 9. PMID 10792042, Free PMC Article
    4. Synthetase recognition determinants of E. coli valine transfer RNA. Horowitz J, et al. Biochemistry, 1999 Jun 15. PMID 10387013
    5. Role of acceptor stem conformation in tRNAVal recognition by its cognate synthetase. Liu M, et al. Nucleic Acids Res, 1997 Dec 15. PMID 9396792, Free PMC Article

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mechanism of discrimination of isoleucyl-tRNA synthetase against nonproteinogenic alpha-aminobutyrate and its fluorinated analogues.
      Title: Mechanism of discrimination of isoleucyl-tRNA synthetase against nonproteinogenic α-aminobutyrate and its fluorinated analogues.
    2. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables aminoacyl-tRNA editing activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aminoacyl-tRNA editing activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables aminoacyl-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables valine-tRNA ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables valine-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables valine-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables valine-tRNA ligase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in aminoacyl-tRNA metabolism involved in translational fidelity IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cytosolic valyl-tRNA aminoacylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of translational fidelity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA aminoacylation for protein translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in valyl-tRNA aminoacylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in valyl-tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within valyl-tRNA aminoacylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    valine--tRNA ligase
    NP_418679.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4480982..4483837 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418679.1 valine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418679.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A7B0E0
      Conserved Domains (1) summary
      PRK05729
      Location:1 → 950
      valS; valyl-tRNA synthetase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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