POSH Plenty of SH3s [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: POSHprovided by FlyBase
Official Full Name: Plenty of SH3sprovided by FlyBase
Primary source: FLYBASE:FBgn0040294
Locus tag: Dmel_CG4909
See related: AllianceGenome:FB:FBgn0040294
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 155116_at; BEST:GM09451; CG4909; Dmel\CG4909; dPOSH; DPOSH; GM09451; l(2)k15815; posh
Summary: Enables protein kinase binding activity and ubiquitin protein ligase activity. Involved in several processes, including determination of adult lifespan; embryonic development via the syncytial blastoderm; and regulation of signal transduction. Predicted to be located in cytosol. Is expressed in several structures, including embryonic dorsal epidermis; foregut primordium; hindgut proper primordium; imaginal disc; and somatic muscle primordium. Orthologous to several human genes including SH3RF3 (SH3 domain containing ring finger 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 54C9-54C9; 2-83 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17568732..17572322)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (13456237..13459827)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E2 enzyme Bruce negatively regulates Hippo signaling through POSH-mediated expanded degradation.
Title: E2 enzyme Bruce negatively regulates Hippo signaling through POSH-mediated expanded degradation.
The pro-apoptotic JNK scaffold protein POSH mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia.
Title: The pro-apoptotic JNK scaffold POSH/SH3RF1 mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia.
The findings establish POSH as a crucial regulator that integrates the signal from the cell surface to negatively regulate Ex-mediated Hippo activation in Drosophila.
Title: POSH regulates Hippo signaling through ubiquitin-mediated expanded degradation.
The data data uncover Rab8, POSH, and TAK1 as regulators of synaptic growth responses and point to recycling endosome as a key compartment for synaptic growth regulation during neurodegenerative processes.
Title: Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.
evidence that the JNK pathway scaffold protein, POSH, is involved in TNF (Eiger) signaling in Drosophila
Title: POSH is involved in Eiger-Basket (TNF-JNK) signaling and embryogenesis in Drosophila.
POSH promotes cell survival in Drosophila and in human rheumatoid arthritis synovial fibroblasts
Title: POSH promotes cell survival in Drosophila and in human RASF cells.
Phenotypic consequences of POSH expression were found to be dependent on Drosophila Nc, the caspase-9 homolog, but only partially on Tak1 and not at all on Slpr and Hep.
Title: POSH misexpression induces caspase-dependent cell death in Drosophila.
activates the JNK/SAPK signaling pathway, and that neural-specific overexpression of DPOSH extends the longevity of flies.
Title: Neural-specific overexpression of drosophila plenty of SH3s (DPOSH) extends the longevity of adult flies.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-band shortening	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in head involution	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of negative regulation of apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hippo signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of JNK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of tumor necrosis factor-mediated signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to peptidoglycan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: plenty of SH3s

Names: CG4909-PA; POSH-PA; Plenty-of-SH3s; plenty of SH3 domains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

17568732..17572322

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079052.4 → NP_523776.1 plenty of SH3s [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523776.1

Status: REVIEWED

UniProtKB/TrEMBL

Q7K4D1, Q9NHP2

Related

FBpp0086007

Conserved Domains (5) summary

cd11783
Location:424 → 477

SH3_SH3RF_3; Third Src Homology 3 domain of SH3 domain containing ring finger 1 (SH3RF1), SH3RF3, and similar domains

cd11785
Location:782 → 836

SH3_SH3RF_C; C-terminal (Fourth) Src Homology 3 domain of SH3 domain containing ring finger 1 (SH3RF1), SH3RF3, and similar domains

cd11787
Location:199 → 251

SH3_SH3RF_2; Second Src Homology 3 domain of SH3 domain containing ring finger proteins

cd00162
Location:11 → 53

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cl17036
Location:139 → 191

SH3; Src Homology 3 domain superfamily