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    oprF outer membrane porin F [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 878442, updated on 11-Sep-2024

    Summary

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    Gene symbol
    oprF
    Gene description
    outer membrane porin F
    Locus tag
    PA1777
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (1921174..1922226)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene hypothetical protein Neighboring gene RNA polymerase sigma factor SigX Neighboring gene uroporphyrin-III C-methyltransferase Neighboring gene assimilatory nitrate reductase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Modeling and simulations of a bacterial outer membrane protein: OprF from Pseudomonas aeruginosa. Khalid S, et al. Proteins, 2006 Apr 1. PMID 16397890
    2. Construction and characterization of Pseudomonas aeruginosa protein F-deficient mutants after in vitro and in vivo insertion mutagenesis of the cloned gene. Woodruff WA, et al. J Bacteriol, 1988 Jun. PMID 2836364, Free PMC Article
    3. Killing from the inside: Intracellular role of T3SS in the fate of Pseudomonas aeruginosa within macrophages revealed by mgtC and oprF mutants. Garai P, et al. PLoS Pathog, 2019 Jun. PMID 31220187, Free PMC Article
    4. Pseudomonas aeruginosa porin OprF exists in two different conformations. Sugawara E, et al. J Biol Chem, 2006 Jun 16. PMID 16595653, Free PMC Article
    5. Factors affecting the folding of Pseudomonas aeruginosa OprF porin into the one-domain open conformer. Sugawara E, et al. mBio, 2010 Oct 19. PMID 20978537, Free PMC Article

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. work highlights T3SS and ExoS, whose expression is modulated by MgtC and OprF, as key players in the intramacrophage life of P. aeruginosa which allow internalized bacteria to lyse macrophages.
      Title: Killing from the inside: Intracellular role of T3SS in the fate of Pseudomonas aeruginosa within macrophages revealed by mgtC and oprF mutants.
    2. OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b.
      Title: Identification of OprF as a complement component C3 binding acceptor molecule on the surface of Pseudomonas aeruginosa.
    3. The highest level was observed for IL-1 and IL-6 after administration of recombinant proteins OprL, OprF, OprF-1, aTox.
      Title: [Pseudomonas aeruginosa recombinant proteins: effect on mice cytokine profile].
    4. LecB binds to the outer membrane protein OprF
      Title: Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
    5. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX.
      Title: Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced.
    6. Prevention of disulfide bond formation in OprF increases its pore-forming activity.
      Title: Factors affecting the folding of Pseudomonas aeruginosa OprF porin into the one-domain open conformer.
    7. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production
      Title: Full virulence of Pseudomonas aeruginosa requires OprF.
    8. Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells.
      Title: [Construction of MyD88-Pseudomonas aeruginosa epitope DNA vaccine and its expression in eukaryotic cells].
    9. Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model.
      Title: Neutrophil elastase mediates innate host protection against Pseudomonas aeruginosa.
    10. analysis of OprF from Pseudomonas aeruginosa
      Title: Modeling and simulations of a bacterial outer membrane protein: OprF from Pseudomonas aeruginosa.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Function Evidence Code Pubs
    enables complement component C3b binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables porin activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within adhesion of symbiont to host IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in outer membrane TAS
    Traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    outer membrane porin F
    NP_250468.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      1921174..1922226
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_250468.1 outer membrane porin F [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_250468.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      F6KQ72
      Conserved Domains (2) summary
      cd07185
      Location:239 → 345
      OmpA_C-like; Peptidoglycan binding domains similar to the C-terminal domain of outer-membrane protein OmpA
      pfam05736
      Location:25 → 184
      OprF; OprF membrane domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P13794.1

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