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    Nfasc neurofascin [ Rattus norvegicus (Norway rat) ]

    Gene ID: 116690, updated on 9-Dec-2024

    Summary

    Official Symbol
    Nfascprovided by RGD
    Official Full Name
    neurofascinprovided by RGD
    Primary source
    RGD:620911
    See related
    EnsemblRapid:ENSRNOG00000030515 AllianceGenome:RGD:620911
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    NF
    Summary
    This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]
    Expression
    Biased expression in Brain (RPKM 154.3) and Muscle (RPKM 7.2) See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See Nfasc in Genome Data Viewer
    Location:
    13q13
    Exon count:
    35
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (46549369..46735989, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (43997223..44183863, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (49335407..49522474, complement)

    Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene RB binding protein 5, histone lysine methyltransferase complex subunit Neighboring gene transmembrane protein 81 Neighboring gene U6 spliceosomal RNA Neighboring gene contactin 2 Neighboring gene transmembrane protein 126A, pseudogene 2 Neighboring gene uncharacterized LOC120096186

    Genomic regions, transcripts, and products

    Expression

    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables cell-cell adhesion mediator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein binding involved in heterotypic cell-cell adhesion ISO
    Inferred from Sequence Orthology
    more info
     
    enables protein domain specific binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in axon guidance IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    acts_upstream_of_or_within axon guidance ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in brain development IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell-cell adhesion IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    acts_upstream_of_or_within clustering of voltage-gated sodium channels ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in heterotypic cell-cell adhesion IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in myelination IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    acts_upstream_of_or_within paranodal junction assembly ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in paranodal junction assembly ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in peripheral nervous system development IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    acts_upstream_of_or_within protein localization to juxtaparanode region of axon ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within protein localization to paranode region of axon ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in protein localization to plasma membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    acts_upstream_of_or_within synapse organization ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within transmission of nerve impulse ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    located_in Schwann cell microvillus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in axon IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in axon IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in axon ISO
    Inferred from Sequence Orthology
    more info
     
    located_in axon initial segment IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in axon initial segment ISO
    Inferred from Sequence Orthology
    more info
     
    located_in dendrite IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in neuron projection IEA
    Inferred from Electronic Annotation
    more info
     
    located_in node of Ranvier IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in node of Ranvier ISO
    Inferred from Sequence Orthology
    more info
     
    located_in paranodal junction ISO
    Inferred from Sequence Orthology
    more info
     
    located_in paranode region of axon ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
     

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001160313.3NP_001153785.2  neurofascin isoform 2 precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556).
      Source sequence(s)
      JAXUCZ010000013
      UniProtKB/TrEMBL
      A6IC61, D3ZW56
      Conserved Domains (7) summary
      cd00063
      Location:639729
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam13882
      Location:10811161
      Bravo_FIGEY; Bravo-like intracellular region
      cd00096
      Location:365369
      Ig; Ig strand B [structural motif]
      cd05731
      Location:263345
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:138228
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:35129
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:349437
      Ig; Immunoglobulin domain
    2. NM_001160314.3NP_001153786.1  neurofascin isoform 1 precursor

      See identical proteins and their annotated locations for NP_001153786.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556).
      Source sequence(s)
      JAXUCZ010000013
      UniProtKB/Swiss-Prot
      P97684, P97685, Q91Z60
      UniProtKB/TrEMBL
      A6IC58
      Related
      ENSRNOP00000057594.4, ENSRNOT00000060871.6
      Conserved Domains (9) summary
      cd05731
      Location:264334
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM)
      cd05875
      Location:59135
      Ig6_hNeurofascin_like; Sixth immunoglobulin (Ig)-like domain of human neurofascin (NF)
      smart00408
      Location:449508
      IGc2; Immunoglobulin C-2 Type
      smart00410
      Location:253333
      IG_like; Immunoglobulin like
      cd00063
      Location:628718
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:528609
      I-set; Immunoglobulin I-set domain
      pfam00041
      Location:10151086
      fn3; Fibronectin type III domain
      pfam13882
      Location:11321216
      Bravo_FIGEY; Bravo-like intracellular region
      cl11960
      Location:140232
      Ig; Immunoglobulin domain
    3. NM_001160315.3NP_001153787.1  neurofascin isoform 4 precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and lacks two segments in the N-terminal and central regions, compared to isoform 1.
      Source sequence(s)
      JAXUCZ010000013
      UniProtKB/TrEMBL
      A0A8I6GIP5, D3ZW56
      Related
      ENSRNOP00000091013.1, ENSRNOT00000110535.2
      Conserved Domains (8) summary
      cd05731
      Location:258328
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM)
      cd05875
      Location:53129
      Ig6_hNeurofascin_like; Sixth immunoglobulin (Ig)-like domain of human neurofascin (NF)
      smart00408
      Location:443502
      IGc2; Immunoglobulin C-2 Type
      smart00410
      Location:247327
      IG_like; Immunoglobulin like
      cd00063
      Location:607697
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:522603
      I-set; Immunoglobulin I-set domain
      pfam13882
      Location:10491133
      Bravo_FIGEY; Bravo-like intracellular region
      cl11960
      Location:134226
      Ig; Immunoglobulin domain
    4. NM_053909.4NP_446361.2  neurofascin isoform 3 precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1.
      Source sequence(s)
      JAXUCZ010000013
      UniProtKB/TrEMBL
      A6IC60, D3ZNW5, D3ZW56
      Conserved Domains (7) summary
      cd00063
      Location:624714
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam13882
      Location:10661146
      Bravo_FIGEY; Bravo-like intracellular region
      cd00096
      Location:365369
      Ig; Ig strand B [structural motif]
      cd05731
      Location:263345
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:138228
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:35129
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:349437
      Ig; Immunoglobulin domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086031.1 Reference GRCr8

      Range
      46549369..46735989 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063271974.1XP_063128044.1  neurofascin isoform X18

      UniProtKB/TrEMBL
      A0A8I6AN44
    2. XM_017598650.3XP_017454139.1  neurofascin isoform X14

      UniProtKB/TrEMBL
      D3ZW56
    3. XM_063271966.1XP_063128036.1  neurofascin isoform X6

      UniProtKB/TrEMBL
      A0A8L2QSS8
    4. XM_039090280.2XP_038946208.1  neurofascin isoform X17

      UniProtKB/TrEMBL
      A0A8I6AN44
      Conserved Domains (7) summary
      cd00063
      Location:729819
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam13882
      Location:10591139
      Bravo_FIGEY; Bravo-like intracellular region
      cd00096
      Location:470474
      Ig; Ig strand B [structural motif]
      cd05731
      Location:368450
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:243333
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:140234
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:454542
      Ig; Immunoglobulin domain
    5. XM_017598649.3XP_017454138.1  neurofascin isoform X13

      UniProtKB/TrEMBL
      A6IC56
    6. XM_017598648.3XP_017454137.1  neurofascin isoform X12

      UniProtKB/TrEMBL
      A6IC57
    7. XM_063271964.1XP_063128034.1  neurofascin isoform X3

      UniProtKB/TrEMBL
      A0A8L2QSS8
    8. XM_063271973.1XP_063128043.1  neurofascin isoform X16

      UniProtKB/TrEMBL
      A0A8I6AN44
      Related
      ENSRNOP00000095471.1, ENSRNOT00000103161.2
    9. XM_017598641.3XP_017454130.1  neurofascin isoform X5

      UniProtKB/TrEMBL
      A0A8L2QSS8
      Conserved Domains (8) summary
      cd00063
      Location:733823
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam00041
      Location:12261298
      fn3; Fibronectin type III domain
      pfam13882
      Location:13441424
      Bravo_FIGEY; Bravo-like intracellular region
      cd00096
      Location:459463
      Ig; Ig strand B [structural motif]
      cd05731
      Location:357439
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:249339
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:146240
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:443531
      Ig; Immunoglobulin domain
    10. XM_063271965.1XP_063128035.1  neurofascin isoform X4

      UniProtKB/TrEMBL
      A0A8L2QSS8
    11. XM_063271972.1XP_063128042.1  neurofascin isoform X15

      UniProtKB/TrEMBL
      A0A8I6AN44
    12. XM_063271971.1XP_063128041.1  neurofascin isoform X9

      UniProtKB/TrEMBL
      A0A8L2QSS8
      Related
      ENSRNOP00000106961.1, ENSRNOT00000159596.1
    13. XM_017598647.3XP_017454136.1  neurofascin isoform X11

      UniProtKB/TrEMBL
      A6IC56
    14. XM_039090275.2XP_038946203.1  neurofascin isoform X2

      UniProtKB/TrEMBL
      A6IC59
      Conserved Domains (8) summary
      cd00063
      Location:750840
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam00041
      Location:12381310
      fn3; Fibronectin type III domain
      pfam13882
      Location:13561436
      Bravo_FIGEY; Bravo-like intracellular region
      cd00096
      Location:476480
      Ig; Ig strand B [structural motif]
      cd05731
      Location:374456
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:249339
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:146240
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:460548
      Ig; Immunoglobulin domain
    15. XM_017598645.3XP_017454134.1  neurofascin isoform X10

      UniProtKB/TrEMBL
      A6IC57
    16. XM_063271967.1XP_063128037.1  neurofascin isoform X7

      UniProtKB/TrEMBL
      A0A8I6AN44
    17. XM_006249727.5XP_006249789.1  neurofascin isoform X1

      UniProtKB/TrEMBL
      A0A8L2QSS8
      Conserved Domains (9) summary
      cd05731
      Location:386456
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM)
      cd05875
      Location:164240
      Ig6_hNeurofascin_like; Sixth immunoglobulin (Ig)-like domain of human neurofascin (NF)
      smart00408
      Location:571630
      IGc2; Immunoglobulin C-2 Type
      smart00410
      Location:375455
      IG_like; Immunoglobulin like
      cd00063
      Location:750840
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:650731
      I-set; Immunoglobulin I-set domain
      pfam00041
      Location:12441315
      fn3; Fibronectin type III domain
      pfam13882
      Location:13611445
      Bravo_FIGEY; Bravo-like intracellular region
      cl11960
      Location:245337
      Ig; Immunoglobulin domain
    18. XM_063271968.1XP_063128038.1  neurofascin isoform X8

      UniProtKB/TrEMBL
      A6IC59
    19. XM_063271969.1XP_063128039.1  neurofascin isoform X8

      UniProtKB/TrEMBL
      A6IC59
    20. XM_039090281.2XP_038946209.1  neurofascin isoform X19

      UniProtKB/TrEMBL
      A0A8I6GIE9
      Conserved Domains (6) summary
      cd00063
      Location:750840
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd00096
      Location:476480
      Ig; Ig strand B [structural motif]
      cd05731
      Location:374456
      Ig3_L1-CAM_like; Third immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains
      cd05845
      Location:249339
      IgI_2_L1-CAM_like; Second immunoglobulin (Ig)-like domain of the L1 cell adhesion molecule (CAM), and similar domains; member of the I-set of Ig superfamily (IgSF) domains
      cd05875
      Location:146240
      IgI_hNeurofascin_like; Immunoglobulin (Ig)-like domain of human neurofascin (NF); member of the I-set of Ig superfamily (IgSF) domains
      cl11960
      Location:460548
      Ig; Immunoglobulin domain