Pou3f3 POU domain, class 3, transcription factor 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pou3f3provided by MGI
Official Full Name: POU domain, class 3, transcription factor 3provided by MGI
Primary source: MGI:MGI:102564
See related: Ensembl:ENSMUSG00000045515 AllianceGenome:MGI:102564
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Brn1; Otf8; Brn-1; Skin1; HST011; urehr2
Summary: Enables HMG box domain binding activity and sequence-specific DNA binding activity. Involved in several processes, including metanephric nephron epithelium development; negative regulation of apoptotic process; and regulation of DNA-templated transcription. Acts upstream of or within several processes, including forebrain development; positive regulation of transcription by RNA polymerase II; and urea transmembrane transport. Located in nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; phalanx; and sensory organ. Orthologous to human POU3F3 (POU class 3 homeobox 3). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: human all
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Genomic context

Location:: 1 B; 1 21.21 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (42734928..42739370)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (42695768..42700210)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion.
Title: Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion.
Therapeutic Potential of POU3F3, a Novel Long Non-coding RNA, Alleviates the Pathogenesis of Osteoarthritis by Regulating the miR-29a- 3p/FOXO3 Axis.
Title: Therapeutic Potential of POU3F3, a Novel Long Non-coding RNA, Alleviates the Pathogenesis of Osteoarthritis by Regulating the miR-29a- 3p/FOXO3 Axis.
The results of the present study confirm the important function of POU3F3 in nephron patterning, especially in development of the the Thick Ascending Limb of the Loop of Henle. Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle.
Title: Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle.
results suggest that a mutually repressive mechanism exists between Brn1/2 and Rorb expression and that the established expression of Brn1/2 and Rorb further specifies those neurons into L2/3 and L4, respectively, during UCP maturation
Title: Mutually repressive interaction between Brn1/2 and Rorb contributes to the establishment of neocortical layer 2/3 and layer 4.
Line Pou3f3L423P is a viable mouse model harboring a homozygous Pou3f3 mutation.
Title: Generation and Standardized, Systemic Phenotypic Analysis of Pou3f3L423P Mutant Mice.
Brn1 predominantly forms homomdimers on MORE cis elements in NS5 mouse neural stem cells
Title: Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells.
Onset of Pou3f3 expression occurs in ventricular zone progenitors, and that Pou3f3 subsequently label neural progeny switching from deep-layer Ctip2+ identity to Satb2+ upper-layer fate as they migrate to proper superficial positions.
Title: POU-III transcription factors (Brn1, Brn2, and Oct6) influence neurogenesis, molecular identity, and migratory destination of upper-layer cells of the cerebral cortex.
Data show that Oct-6 normally represses Wnt1 expression in the early diencephalon and replacement by Brn-1 as a weaker inhibitor is no longer sufficient to maintain the necessary level of repression in the mouse mutant.
Title: Replacement of related POU transcription factors leads to severe defects in mouse forebrain development.
Sox2 and SF1 may mediate basal nestin expression in undifferentiated cells, whereas Sox2, Brn1, and Brn2 bind to the enhancer in neural progenitor cells.
Title: Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.
These results suggest that Brn1 is essential for both the development and function of the nephron in the kidney.
Title: Crucial roles of Brn1 in distal tubule formation and function in mouse kidney.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1Mit228 (e-PCR), detects polymorphism
- UniSTS:128571

D9M1Mit228 (e-PCR)
- UniSTS:128572

D1Mit228.1 (e-PCR), detects polymorphism
- UniSTS:128572

NoName (e-PCR)
- UniSTS:484977

Pou3f3 (e-PCR), detects polymorphism
- UniSTS:471183

D1S3688 (e-PCR)
- UniSTS:474266

Pou3f3 (e-PCR), detects polymorphism
- UniSTS:547295

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info	PubMed
enables HMG box domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cerebral cortex radially oriented cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within chemical homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within forebrain ventricular zone progenitor cell division	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within kidney development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metanephric DCT cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metanephric ascending thin limb development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metanephric loop of Henle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metanephric macula densa development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metanephric thick ascending limb development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within urea transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: POU domain, class 3, transcription factor 3

Names: OTF-8; brain-1; brain-specific homeobox/POU domain protein 1; oct-8; octamer-binding protein 8; octamer-binding transcription factor 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008900.3 → NP_032926.2 POU domain, class 3, transcription factor 3

See identical proteins and their annotated locations for NP_032926.2

Status: VALIDATED

Source sequence(s)

AC122898

Consensus CDS

CCDS14917.1

UniProtKB/Swiss-Prot

P31361

Related

ENSMUSP00000052088.3, ENSMUST00000054883.4

Conserved Domains (2) summary

smart00352
Location:311 → 385

POU; Found in Pit-Oct-Unc transcription factors

pfam00046
Location:406 → 460

Homeobox; Homeobox domain