Glis3 GLIS family zinc finger 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Glis3provided by MGI
Official Full Name: GLIS family zinc finger 3provided by MGI
Primary source: MGI:MGI:2444289
See related: Ensembl:ENSMUSG00000052942 AllianceGenome:MGI:2444289
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4833409N03Rik; E330013K21Rik
Summary: This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein which contains multiple C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the transcriptional regulation of insulin. It is thought to enhance GLI-RE-dependent transcription by binding to the GLI-RE consensus sequence (GACCACCCAC). Mutations in a similar gene in human have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
Expression: Broad expression in kidney adult (RPKM 6.0), ovary adult (RPKM 1.9) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C1; 19 22.88 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (28236251..28657684, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (28258851..28680265, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GLIS3 expression in the thyroid gland in relation to TSH signaling and regulation of gene expression.
Title: GLIS3 expression in the thyroid gland in relation to TSH signaling and regulation of gene expression.
GLIS3: A Critical Transcription Factor in Islet beta-Cell Generation.
Title: GLIS3: A Critical Transcription Factor in Islet β-Cell Generation.
Identification of a novel lncRNA (G3R1) regulated by GLIS3 in pancreatic beta-cells.
Title: Identification of a novel lncRNA (G3R1) regulated by GLIS3 in pancreatic β-cells.
an unexpected role for GLIS3 in the development of male germ cells
Title: Loss of Glis3 causes dysregulation of retrotransposon silencing and germ cell demise in fetal mouse testis.
study identifies GLIS3 as a key regulator of TSH/TSHR-mediated thyroid hormone biosynthesis and proliferation of thyroid follicular cells and uncovers a mechanism by which GLIS3 deficiency causes neonatal hypothyroidism and prevents goiter development.
Title: GLIS3 is indispensable for TSH/TSHR-dependent thyroid hormone biosynthesis and follicular cell proliferation.
The observations suggest that GLIS3 promotes the gonocyte-to- spermatogonial stem cells transition and is a critical regulator of the dynamics of early postnatal spermatogenesis.
Title: Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis.
Study demonstrates that Glis3 protein exhibits a temporal and cell type-specific pattern of expression during embryonic and neonatal pancreas development that is consistent with a regulatory role for Glis3 in promoting endocrine progenitor generation, regulating insulin and Ppy expression in beta and Ppy expression in beta and PP cells, respectively, and duct morphogenesis.
Title: The Spatiotemporal Pattern of Glis3 Expression Indicates a Regulatory Function in Bipotent and Endocrine Progenitors during Early Pancreatic Development and in Beta, PP and Ductal Cells.
Glis3 plays a pivotal role in the transcriptional regulation of insulin.
Title: The Krüppel-like protein Gli-similar 3 (Glis3) functions as a key regulator of insulin transcription.
beta cell-specific inactivation of Glis3 in adult mice downregulates insulin expression, leading to hyperglycaemia and subsequently enhanced beta cell apoptosis
Title: Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.
The results indicate that GLIS3 controls fetal islet differentiation via direct transactivation of Neurog3, a perturbation
Title: The Krüppel-like zinc finger protein GLIS3 transactivates neurogenin 3 for proper fetal pancreatic islet differentiation in mice.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU042950 (e-PCR)
- UniSTS:160819

Results_ER_8_28_95_2_42.seq (e-PCR)
- UniSTS:125573

D19Dcr10 (e-PCR), detects polymorphism
- UniSTS:507541

D19Dcr9 (e-PCR), detects polymorphism
- UniSTS:507562

D19Mit98 (e-PCR), detects polymorphism
- UniSTS:128434

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: zinc finger protein GLIS3

Names: GLI-similar 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001305671.1 → NP_001292600.1 zinc finger protein GLIS3 isoform 2

See identical proteins and their annotated locations for NP_001292600.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an internal exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (2) that has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC118934, AK087731, BP768786, CK640192, DQ871027

UniProtKB/TrEMBL

A2VDF9

Conserved Domains (3) summary

sd00017
Location:293 → 315

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:307 → 334

zf-H2C2_2; Zinc-finger double domain

pfam15909
Location:293 → 380

zf-C2H2_8; C2H2-type zinc ribbon
NM_001404124.1 → NP_001391053.1 zinc finger protein GLIS3 isoform 1

Status: REVIEWED

Source sequence(s)

AC116137, AC116863, AC118934, AC161521

UniProtKB/TrEMBL

Q0GE24
NM_175459.6 → NP_780668.3 zinc finger protein GLIS3 isoform 1

See identical proteins and their annotated locations for NP_780668.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC118934, AK087731, BP768786, CK640192, DQ871027

Consensus CDS

CCDS37947.1

UniProtKB/TrEMBL

A2VDF9, Q0GE24

Related

ENSMUSP00000124635.2, ENSMUST00000162022.8

Conserved Domains (3) summary

PHA03247
Location:612 → 861

PHA03247; large tegument protein UL36; Provisional

COG5189
Location:558 → 652

SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]

sd00017
Location:569 → 591

ZF_C2H2; C2H2 Zn finger [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

28236251..28657684 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006526985.5 → XP_006527048.1 zinc finger protein GLIS3 isoform X2

See identical proteins and their annotated locations for XP_006527048.1

UniProtKB/Swiss-Prot

Q6XP49, Q8BI60

UniProtKB/TrEMBL

A2VDF9

Conserved Domains (3) summary

PHA03247
Location:457 → 706

PHA03247; large tegument protein UL36; Provisional

COG5189
Location:403 → 497

SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]

sd00017
Location:414 → 436

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_036161561.1 → XP_036017454.1 zinc finger protein GLIS3 isoform X3

Conserved Domains (2) summary

COG5048
Location:565 → 672

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:569 → 591

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_036161562.1 → XP_036017455.1 zinc finger protein GLIS3 isoform X4

Related

ENSMUSP00000108231.3, ENSMUST00000112612.9

Conserved Domains (2) summary

COG5189
Location:558 → 652

SFP1; Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning]

sd00017
Location:569 → 591

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_006526987.5 → XP_006527050.1 zinc finger protein GLIS3 isoform X5

Conserved Domains (2) summary

COG5048
Location:565 → 621

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:569 → 591

ZF_C2H2; C2H2 Zn finger [structural motif]