Limk1 LIM domain kinase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Limk1provided by RGD
Official Full Name: LIM domain kinase 1provided by RGD
Primary source: RGD:62055
See related: EnsemblRapid:ENSRNOG00000001470 AllianceGenome:RGD:62055
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable heat shock protein binding activity and protein serine/threonine kinase activity. Involved in regulation of modification of postsynaptic actin cytoskeleton. Located in axonal growth cone and perinuclear region of cytoplasm. Is active in glutamatergic synapse. Orthologous to human LIMK1 (LIM domain kinase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 42.9), Adrenal (RPKM 25.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12q12

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (27663177..27697085)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (22026697..22060605)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (25036630..25070538)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Circ-Zfp644 acts as a pro-hypertrophic mediator in an Ang-II induced in vitro myocardial hypertrophy model.
Title: Circ-Zfp644 acts as a pro-hypertrophic mediator in an Ang-II induced in vitro myocardial hypertrophy model.
Cofilin Inhibition by Limk1 Reduces Rod Formation and Cell Apoptosis after Ischemic Stroke.
Title: Cofilin Inhibition by Limk1 Reduces Rod Formation and Cell Apoptosis after Ischemic Stroke.
We demonstrated that LIMK1, as one target of miR-27b, might mediate sevoflurane-induced synapse deficits in the hippocampus. These results emphasize the potential role of LIMK1 in cognitive dysfunction in rats.
Title: Sevoflurane induces cognitive impairments via the MiR-27b/LIMK1-signaling pathway in developing rats.
MiR-134-mediated LIM domain kinase was involved in Regulates Hippocampal Synaptic Plasticity via miR-134-mediated LIMK1 function in rats with ischemic stroke induced hippocampal synaptic plasticity, which served as a contributor to improving learning and memory during the recovery stage of ischemic stroke.
Title: Electroacupuncture Regulates Hippocampal Synaptic Plasticity via miR-134-Mediated LIMK1 Function in Rats with Ischemic Stroke.
Data indicate that LIM motif-containing protein kinases LIMK1 and LIMK2-dependent acin rearrangement is involved in the development of complete Freund's adjuvant (CFA)-induced inflammatory pain.
Title: LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.
the functional relevance of the LRAP25-MRCK complex in LIMK1-cofilin signaling and the importance of LRAP adaptors as key determinants of MRCK cellular localization and downstream specificities.
Title: Adaptor protein LRAP25 mediates myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) regulation of LIMK1 protein in lamellipodial F-actin dynamics.
the CaMKIIbeta phosphorylates LIMK1 at Thr-508 in the kinase domain and activates the cofilin-phosphorylating activity of LIMK1.
Title: CaMKIIβ-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis.
BDNF-induced TrkB dimerization led to LIMK1 dimerization and transphosphorylation independent of TrkB kinase activity, which could further enhance the activation and stabilization of LIMK1
Title: LIM kinase 1 (LIMK1) interacts with tropomyosin-related kinase B (TrkB) and Mediates brain-derived neurotrophic factor (BDNF)-induced axonal elongation.
Linkage of estrogen communication to cofilin via LIMK provides functionality to the LIMK immunoreactivity that has been observed in the brain in vivo.
Title: LIM kinase mediates estrogen action on the actin depolymerization factor Cofilin.
both LIMK1-mediated phosphorylation and SSH1/SSH2-mediated dephosphorylation of cofilin/ADF are critical for the migration and invasion of tumor cells
Title: Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM kinase.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Limk1 (e-PCR), detects polymorphism
- UniSTS:527404

RH142289 (e-PCR)
- UniSTS:231536

UniSTS:230793 (e-PCR)
- UniSTS:230793

Limk1 (e-PCR), detects polymorphism
- UniSTS:230793

G48114 (e-PCR)
- UniSTS:95224

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables heat shock protein binding	IEA Inferred from Electronic Annotation more info
enables heat shock protein binding	ISO Inferred from Sequence Orthology more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in axon extension	IEA Inferred from Electronic Annotation more info
acts_upstream_of axon extension	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament bundle assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of stress fiber assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of stress fiber assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of modification of postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of modification of postsynaptic actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress fiber assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of stress fiber assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axonal growth cone	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in male germ cell nucleus	IEA Inferred from Electronic Annotation more info
located_in male germ cell nucleus	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: LIM domain kinase 1

Names: LIM motif-containing protein kinase 1; LIM-domain containing, protein kinase 1; LIMK-1

NP_113915.2

EC 2.7.11.1

XP_006249247.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031727.2 → NP_113915.2 LIM domain kinase 1

See identical proteins and their annotated locations for NP_113915.2

Status: VALIDATED

Source sequence(s)

AC091000, AW529884, BF565872, CB691076, CB702103, CB726578, CV112377

UniProtKB/Swiss-Prot

P53669

UniProtKB/TrEMBL

A6J0H7, G3V663

Related

ENSRNOP00000001996.2, ENSRNOT00000001996.6

Conserved Domains (5) summary

cd09462
Location:5 → 77

LIM1_LIMK1; The first LIM domain of LIMK1 (LIM domain Kinase 1)

cd09464
Location:84 → 138

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:342 → 604

TyrKc; Tyrosine kinase, catalytic domain

cd14221
Location:345 → 611

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:165 → 255

PDZ; PDZ domain (Also known as DHR or GLGF)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

27663177..27697085

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006249185.5 → XP_006249247.1 LIM domain kinase 1 isoform X1

UniProtKB/TrEMBL

A0A0G2K4K5, A0A8I6GL45

Related

ENSRNOP00000094337.1, ENSRNOT00000118532.2

Conserved Domains (5) summary

cd09464
Location:50 → 104

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:308 → 570

TyrKc; Tyrosine kinase, catalytic domain

cd14221
Location:311 → 577

STKc_LIMK1; Catalytic domain of the Serine/Threonine Kinase, LIM domain kinase 1

pfam00595
Location:131 → 221

PDZ; PDZ domain (Also known as DHR or GLGF)

cl02475
Location:17 → 43

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers