UBE2N ubiquitin conjugating enzyme E2 N [Homo sapiens (human)] - Gene

Summary

Official Symbol: UBE2Nprovided by HGNC
Official Full Name: ubiquitin conjugating enzyme E2 Nprovided by HGNC
Primary source: HGNC:HGNC:12492
See related: Ensembl:ENSG00000177889 MIM:603679; AllianceGenome:HGNC:12492
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: UBC13; UbcH13; UBCHBEN; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in testis (RPKM 18.8), brain (RPKM 12.2) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 12q22

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (93405684..93441947, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (93386662..93422932, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (93799460..93835723, complement)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppressing UBE2N ameliorates Alzheimer's disease pathology through the clearance of amyloid beta.
Title: Suppressing UBE2N ameliorates Alzheimer's disease pathology through the clearance of amyloid beta.
UBE2N promotes cell viability and glycolysis by promoting Axin1 ubiquitination in prostate cancer cells.
Title: UBE2N promotes cell viability and glycolysis by promoting Axin1 ubiquitination in prostate cancer cells.
SET7 methylates the deubiquitinase OTUB1 at Lys [122] to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis.
Title: SET7 methylates the deubiquitinase OTUB1 at Lys (122) to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis.
Blocking UBE2N abrogates oncogenic immune signaling in acute myeloid leukemia.
Title: Blocking UBE2N abrogates oncogenic immune signaling in acute myeloid leukemia.
The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels.
Title: The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels.
Uev1A amino terminus stimulates poly-ubiquitin chain assembly and is required for NF-kappaB activation.
Title: Uev1A amino terminus stimulates poly-ubiquitin chain assembly and is required for NF-κB activation.
Functional interaction of ubiquitin ligase RNF167 with UBE2D1 and UBE2N promotes ubiquitination of AMPA receptor.
Title: Functional interaction of ubiquitin ligase RNF167 with UBE2D1 and UBE2N promotes ubiquitination of AMPA receptor.
Ubc13-Mms2 cooperates with a family of RING E3 proteins in budding yeast membrane protein sorting.
Title: Ubc13-Mms2 cooperates with a family of RING E3 proteins in budding yeast membrane protein sorting.
Inhibiting ubiquitin conjugating enzyme E2 N by microRNA-590-3p reduced cell growth of cervical carcinoma.
Title: Inhibiting ubiquitin conjugating enzyme E2 N by microRNA-590-3p reduced cell growth of cervical carcinoma.
Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler.
Title: Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler.

Submit: New GeneRIF Correction See all GeneRIFs (53)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Many sequence variants affecting diversity of adult human height. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
capsid	gag	Heterodimeric E2 UBC13-UEV1A and the arginine 63-linked ubiquitin chains play a role in TRIM5alpha-mediated signaling, which leads to capsid-specific restriction	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BCD1455 (e-PCR)
- UniSTS:73279

RH80774 (e-PCR)
- UniSTS:88923

NIB1585 (e-PCR)
- UniSTS:65475

WI-19812 (e-PCR)
- UniSTS:29646

STS-N63414 (e-PCR)
- UniSTS:51928

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	HDA more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin conjugating enzyme activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin conjugating enzyme activity	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin conjugating enzyme activity	TAS Traceable Author Statement more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-protein transferase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in antiviral innate immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of double-strand break repair	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of postreplication repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein monoubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	TAS Traceable Author Statement more info	PubMed
involved_in regulation of DNA repair	TAS Traceable Author Statement more info	PubMed
involved_in regulation protein catabolic process at postsynapse	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of UBC13-MMS2 complex	IDA Inferred from Direct Assay more info	PubMed
part_of UBC13-MMS2 complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IC Inferred by Curator more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in postsynapse	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin conjugating enzyme complex	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 N

Names: E2 ubiquitin-conjugating enzyme N; bendless-like ubiquitin conjugating enzyme; epididymis secretory protein Li 71; ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-protein ligase N; yeast UBC13 homolog

NP_003339.1

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003348.4 → NP_003339.1 ubiquitin-conjugating enzyme E2 N

See identical proteins and their annotated locations for NP_003339.1

Status: REVIEWED

Source sequence(s)

AC025260, AK098233, BC000396, CB243041

Consensus CDS

CCDS31875.1

UniProtKB/Swiss-Prot

P61088, Q16781, Q53Y81

UniProtKB/TrEMBL

V9HW41

Related

ENSP00000316176.2, ENST00000318066.7

Conserved Domains (1) summary

cl00154
Location:4 → 151

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...