pasha partner of drosha [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pashaprovided by FlyBase
Official Full Name: partner of droshaprovided by FlyBase
Primary source: FLYBASE:FBgn0039861
Locus tag: Dmel_CG1800
See related: AllianceGenome:FB:FBgn0039861
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cg1800; CG1800; DGCR8; dmDGCR8; Dmel\CG1800; Pasha; PASHA
Summary: Predicted to enable double-stranded RNA binding activity and primary miRNA binding activity. Involved in several processes, including germarium-derived egg chamber formation; imaginal disc growth; and regulatory ncRNA-mediated gene silencing. Located in nucleus. Part of microprocessor complex. Is expressed in neurons. Human ortholog(s) of this gene implicated in alcohol dependence. Orthologous to human DGCR8 (DGCR8 microprocessor complex subunit). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 100D1-100D1; 3-102 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (31617977..31622217)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (27443699..27447939)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Drosha and Pasha are required for coordinated development of somatic and germ cell precursors in the larval ovary. Loss of Drosha or Pasha function perturbs somatic precursor development, causing defects in formation of terminal filaments.
Title: MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis.
report clear phenotypic differences between drosha and pasha/dgcr8 null alleles in two postembryonic lineages in the Drosophila brain
Title: Drosha-independent DGCR8/Pasha pathway regulates neuronal morphogenesis.
Excess Drosha cleaves pasha/DGCR8 primary transcripts and leads to a reduction in pasha/DGCR8 mRNA levels and Pasha/DGCR8 synthesis.
Title: Genome-wide identification of targets of the drosha-pasha/DGCR8 complex.
Pasha is essential for the biogenesis of canonical miRNAs but is dispensable for the processing and function of mirtron-derived regulatory RNAs.
Title: A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways.
role for Pasha in miRNA maturation and miRNA-mediated gene regulation
Title: Processing of primary microRNAs by the Microprocessor complex.
DGCR8 is required for the maturation of miRNA primary transcripts.
Title: The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables primary miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in germarium-derived female germ-line cyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in primary miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in primary miRNA processing	IEA Inferred from Electronic Annotation more info
involved_in primary miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of microprocessor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of microprocessor complex	IDA Inferred from Direct Assay more info	PubMed
part_of microprocessor complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: partner of drosha

Names: CG1800-PA; CG1800-PB; CG1800-PC; DiGeorge critical region 8; pasha; pasha-PA; pasha-PB; pasha-PC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

31617977..31622217

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_143622.3 → NP_651879.1 partner of drosha, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_651879.1

Status: REVIEWED

UniProtKB/TrEMBL

H5V8G4, Q9V9V7

Related

FBpp0085181

Conserved Domains (2) summary

smart00358
Location:514 → 549

DSRM; Double-stranded RNA binding motif

cd00048
Location:370 → 434

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001276221.1 → NP_001263150.1 partner of drosha, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263150.1

Status: REVIEWED

UniProtKB/TrEMBL

H5V8G4, Q9V9V7

Conserved Domains (2) summary

smart00358
Location:514 → 549

DSRM; Double-stranded RNA binding motif

cd00048
Location:370 → 434

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001276220.1 → NP_001263149.1 partner of drosha, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263149.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KI70, H5V8G4

Conserved Domains (3) summary

smart00456
Location:93 → 122

WW; Domain with 2 conserved Trp (W) residues

smart00358
Location:452 → 487

DSRM; Double-stranded RNA binding motif

cd00048
Location:308 → 372

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...