TPK1 cAMP-dependent protein kinase catalytic subunit TPK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TPK1
Official Full Name: cAMP-dependent protein kinase catalytic subunit TPK1
Primary source: SGD:S000003700
Locus tag: YJL164C
See related: AllianceGenome:SGD:S000003700; FungiDB:YJL164C; VEuPathDB:YJL164C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PKA1; SRA3
Summary: Enables cAMP-dependent protein kinase activity. Involved in several processes, including intracellular signal transduction; negative regulation of cytoplasmic mRNA processing body assembly; and protein import into nucleus. Located in chromatin; cytoplasm; and nucleus. Part of cAMP-dependent protein kinase complex. Human ortholog(s) of this gene implicated in adrenal cortical adenoma; congestive heart failure; fibrolamellar carcinoma; platelet-type bleeding disorder 19; and primary pigmented nodular adrenocortical disease 4. Orthologous to several human genes including PRKACA (protein kinase cAMP-activated catalytic subunit alpha); PRKACB (protein kinase cAMP-activated catalytic subunit beta); and PRKACG (protein kinase cAMP-activated catalytic subunit gamma). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TPK1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (109966..111159, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast.
Title: Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast.
Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair.
Title: Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair.
GRX deletion decreased the expression of stress-response proteins, leading to increased cellular reactive oxygen species accumulation and, subsequently, intracellular acidification.Ras/PKA activation decreased stress resistance and increased biosynthesis, requiring yeast cells to grow under unfavorable conditions and resulting in a shortened Chronological Life Span
Title: Glutaredoxin Deletion Shortens Chronological Life Span in Saccharomyces cerevisiae via ROS-Mediated Ras/PKA Activation.
These results show that rapid activation of the TOR pathway by amino acids is not involved in rapid activation of the PKA pathway and that effects of Gcn2 inactivation as well as leu2 auxotrophy all act independently of the TOR pathway.
Title: The nutrient transceptor/PKA pathway functions independently of TOR and responds to leucine and Gcn2 in a TOR-independent manner.
Long non-coding RNA IRT1 transcription is repressed when nutrients are ample and target of rapamycin complex I (TORC1) and protein kinase A (PKA) are active.
Title: Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA.
protein kinase A (PKA) and target of rapamycin complex I (TORC1) signalling mediate nutrient regulation of IME1 expression.
Title: Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability.
The role of Sir2 for dietary restriction-mediated lifespan extension depends on cAMP-protein kinase A (PKA) and casein kinase 2 (CK2) signaling in yeast.
Title: Sir2 phosphorylation through cAMP-PKA and CK2 signaling inhibits the lifespan extension activity of Sir2 in yeast.
Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression.
Title: The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.
results describe that an autophosphorylation mechanism on Tpk1 controls PKA activity in response to glucose availability
Title: Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae.
Trehalase activation is associated with phosphorylation of typical PKA sites.
Title: In vivo phosphorylation of Ser21 and Ser83 during nutrient-induced activation of the yeast protein kinase A (PKA) target trehalase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables cAMP-dependent protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Ras protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cytoplasmic mRNA processing body assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein kinase A signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of cAMP-dependent protein kinase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cAMP-dependent protein kinase catalytic subunit TPK1

NP_012371.2

EC 2.7.11.11

cAMP-dependent protein kinase catalytic subunit; role in nutrient-stimulated growth via Ras-cAMP signaling; inhibited by regulatory subunit Bcy1p in cAMPs absence; phosphorylates and inhibits Whi3p to promote G1/S phase passage; phosphorylates Nej1p to regulate nonhomologous end joining (NHEJ); impairs mitochondrial import of pre-Tom40p via phosphorylation under non-respiratory conditions; partially redundant with Tpk2p and Tpk3p; human homolog PRKACB regulates NHEJ via NHEJ1 phosphorylation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

109966..111159 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181597.2 → NP_012371.2 TPA: cAMP-dependent protein kinase catalytic subunit TPK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012371.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW23, P06244, P11595, Q2VQW1

UniProtKB/TrEMBL

B3LPV9, B5VL21, C7GRZ2, C8ZB55, N1P906

Conserved Domains (1) summary

cd05580
Location:85 → 375

STKc_PKA_like; Catalytic subunit of the Serine/Threonine Kinases, cAMP-dependent protein kinases