GGA2 phosphatidylinositol 4-phosphate-binding protein [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GGA2
Official Full Name: phosphatidylinositol 4-phosphate-binding protein
Primary source: SGD:S000001150
Locus tag: YHR108W
See related: AllianceGenome:SGD:S000001150; FungiDB:YHR108W; VEuPathDB:YHR108W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidylinositol-4-phosphate binding activity and ubiquitin binding activity. Involved in Golgi to endosome transport; Golgi to vacuole transport; and protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Located in trans-Golgi network. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to several human genes including GGA1 (golgi associated, gamma adaptin ear containing, ARF binding protein 1) and GGA2 (golgi associated, gamma adaptin ear containing, ARF binding protein 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GGA2 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (328303..330060)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of Gga2-Pik1 binding resulted in reduced Pik1 localization and delayed accumulation of phosphatidylinositol 4-phosphate to the trans-Golgi network.
Title: Conserved role for Gga proteins in phosphatidylinositol 4-kinase localization to the trans-Golgi network.
Direct binding of the Kex2p cytosolic tail to the VHS domain of yeast Gga2p facilitates trans-Golgi network to prevacuolar compartment transport and is regulated by phosphorylation.
Title: Direct binding of the Kex2p cytosolic tail to the VHS domain of yeast Gga2p facilitates TGN to prevacuolar compartment transport and is regulated by phosphorylation.
Data show that Gga2p binds directly to the Gga2p binds directly to the trans-Golgi network (TGN) PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment.
Title: Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network.
Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5
Title: Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .
Ubiquitin binding by Gga2 was not required for the trans-Golgi network to endosome trafficking of Arn1, but it was required for sorting of Arn1 into the vacuole.
Title: Gga2 mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1 from the trans-Golgi network to the vacuole.
These data provide an explanation for the trans-Golgi network-specific membrane recruitment of Gga2p.
Title: The clathrin adaptor Gga2p is a phosphatidylinositol 4-phosphate effector at the Golgi exit.
clathrin adaptor Gga2 and ubiquitination by the Rsp5 ubiquitin ligase are required for trafficking of Arn1
Title: GGA2- and ubiquitin-dependent trafficking of Arn1, the ferrichrome transporter of Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to endosome transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi to endosome transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Golgi to vacuole transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi to vacuole transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Golgi to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytosolic transport	IEA Inferred from Electronic Annotation more info
involved_in endosomal transport	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuolar transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in trans-Golgi network	IBA Inferred from Biological aspect of Ancestor more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphatidylinositol 4-phosphate-binding protein

NP_011976.1

Protein that regulates Arf1p and Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; K63-specific Ub chain binding receptor with moderate selectivity; homologous to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

328303..330060

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179238.1 → NP_011976.1 TPA: phosphatidylinositol 4-phosphate-binding protein [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011976.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DL58, P38817

UniProtKB/TrEMBL

A6ZT16, B3LSM3, B5VK43, C8Z9Q1, N1P146

Conserved Domains (4) summary

cd16998
Location:26 → 164

VHS_GGA_fungi; VHS (Vps27/Hrs/STAM) domain of fungal GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) proteins

pfam02883
Location:475 → 583

Alpha_adaptinC2; Adaptin C-terminal domain

pfam18308
Location:199 → 233

GGA_N-GAT; GGA N-GAT domain

cd14235
Location:235 → 324

GAT_GGA_fungi; canonical GAT domain found in fungal ADP-ribosylation factor (Arf)-binding proteins (GGAs)