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    putative alanine--tRNA ligase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855688, updated on 17-Dec-2024

    Summary

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    Official Full Name
    putative alanine--tRNA ligase
    Primary source
    SGD:S000004985
    Locus tag
    YNL040W
    See related
    AllianceGenome:SGD:S000004985; FungiDB:YNL040W; VEuPathDB:YNL040W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables L-seryl-tRNA(Thr) hydrolase activity and Ser-tRNA(Ala) hydrolase activity. Involved in aminoacyl-tRNA metabolism involved in translational fidelity. Located in cytoplasm. Orthologous to human PTGES3L-AARSD1 (PTGES3L-AARSD1 readthrough) and AARSD1 (alanyl-tRNA synthetase domain containing 1). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    Location:
    chromosome: XIV
    Exon count:
    1
    Sequence:
    Chromosome: XIV; NC_001146.8 (553380..554750)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Golgi transport complex subunit COG6 Neighboring gene uncharacterized protein Neighboring gene transcription factor TFIIIB subunit BDP1 Neighboring gene phosphatidylinositol N-acetylglucosaminyltransferase GPI15

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Eukaryotic AlaX provides multiple checkpoints for quality and quantity of aminoacyl-tRNAs in translation. Li ZH, et al. Nucleic Acids Res, 2024 Jul 22. PMID 38869066, Free PMC Article
    2. A global investigation of gene deletion strains that affect premature stop codon bypass in yeast, Saccharomyces cerevisiae. Samanfar B, et al. Mol Biosyst, 2014 Apr. PMID 24535059
    3. Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Guo M, et al. Nature, 2009 Dec 10. PMID 20010690, Free PMC Article
    4. Fungal BLAST and Model Organism BLASTP Best Hits: new comparison resources at the Saccharomyces Genome Database (SGD). Balakrishnan R, et al. Nucleic Acids Res, 2005 Jan 1. PMID 15608219, Free PMC Article
    5. Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames. Brachat A, et al. Yeast, 2000 Feb. PMID 10649453

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. deletion of YNL040W increased the rate of premature stop codon bypass and reduced the rate of protein synthesis connecting its activity to the process of protein biosynthesis.
      Title: A global investigation of gene deletion strains that affect premature stop codon bypass in yeast, Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables L-seryl-tRNA(Thr) hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables Ser-tRNA(Ala) hydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables Ser-tRNA(Ala) hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aminoacyl-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in aminoacyl-tRNA metabolism involved in translational fidelity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in aminoacyl-tRNA metabolism involved in translational fidelity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of translational fidelity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 

    General protein information

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    Preferred Names
    putative alanine--tRNA ligase
    NP_014358.3
    • Ser-tRNA(Ala) hydrolase; hydrolyzes Ser-mischarged cytoplasmic and mitochondrial tRNAs; regulates translational fidelity based on Ala- and Thr-to-Ser misincorporation in the null mutant; trans-editing factor for alanyl- and threonyl-tRNA synthetases and seryl-tRNA synthetase (SerRS) via hydrolysis; null mutant displays decreased translation rate and increased readthrough of premature stop codons; (GFP)-fusion protein localizes to the cytoplasm; homologous to human trans-editing factor AARSD

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      553380..554750
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182879.3 → NP_014358.3  TPA: putative alanine--tRNA ligase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014358.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W1D9, P53960
      UniProtKB/TrEMBL
      B5VQZ7, G2WM63, N1NZK3
      Conserved Domains (1) summary
      COG2872
      Location:11 → 281
      AlaX; Ser-tRNA(Ala) deacylase AlaX (editing enzyme) [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53960.1

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