SUI1 translation initiation factor eIF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SUI1
Official Full Name: translation initiation factor eIF1
Primary source: SGD:S000005188
Locus tag: YNL244C
See related: AllianceGenome:SGD:S000005188; FungiDB:YNL244C; VEuPathDB:YNL244C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MOF2; RFR1
Summary: Enables ribosomal small subunit binding activity; translation initiation factor activity; and translation initiation factor binding activity. Involved in formation of translation preinitiation complex; maintenance of translational fidelity; and regulation of translational fidelity. Part of eukaryotic 43S preinitiation complex and multi-eIF complex. Orthologous to human EIF1 (eukaryotic translation initiation factor 1) and EIF1B (eukaryotic translation initiation factor 1B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SUI1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (187170..187496, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eIF2beta or eIF1 substitutions disrupting these contacts increase initiation at UUG codons, and compound substitutions also derepress translation of GCN4, indicating slower TC recruitment.
Title: A network of eIF2β interactions with eIF1 and Met-tRNAi promotes accurate start codon selection by the translation preinitiation complex.
Thus, electrostatic interactions involving the eIF1A N-terminal tail stabilize the closed conformation and promote utilization of suboptimal start codons.
Title: eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast.
eIF1 Loop 2 interactions with Met-tRNAi control the accuracy of start codon selection by the scanning preinitiation complex.
Title: eIF1 Loop 2 interactions with Met-tRNA(i) control the accuracy of start codon selection by the scanning preinitiation complex.
The GTPase-activating protein eIF5 and beta-subunit of eIF2 promote transcriptional accuracy by controlling eIF1 dissociation and the stability of ternary complex binding to the preinitiation complex.
Title: Enhanced eIF1 binding to the 40S ribosome impedes conformational rearrangements of the preinitiation complex and elevates initiation accuracy.
substituting Lys-60 in helix alpha1, or either Lys-37 or Arg-33 in beta-hairpin loop-1, impairs binding of yeast eIF1 to 40 S.eIF1A complexes in vitro, and it confers increased initiation at UUG codons (Sui(-) phenotype)
Title: β-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo.
eIF1 autoregulates translation in yeast and discrimination against poor sequence context in vivo depends on specific domains and residues in eIF1, eIF1A, and eIF2beta
Title: Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons.
Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.
Title: Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.
Data show that start codon recognition results in a conformational change and release of eIF1 from the ribosome.
Title: A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon.
EIF-1 can form the multifactor complex with the ribosome 40S subunit.
Title: Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex.
Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA.
Title: The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribosomal small subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribosomal small subunit binding	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor binding	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in formation of translation preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
involved_in formation of translation preinitiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of translational fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulation of translational fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of eukaryotic 43S preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic 43S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of multi-eIF complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF1

NP_014155.1

Translation initiation factor eIF1; component of complex involved in recognition of initiator codon; modulates translation accuracy at initiation phase by discriminating against suboptimal initiation sites to prevent excessive uORF translation genome-wide

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

187170..187496 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183082.1 → NP_014155.1 TPA: translation initiation factor eIF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014155.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0U9, P32911

UniProtKB/TrEMBL

A6ZRJ9, B3LP83, C7GPB7, C8ZFZ2, G2WLM2, N1NY10

Conserved Domains (1) summary

TIGR01160
Location:2 → 108

SUI1_MOF2; translation initiation factor SUI1, eukaryotic