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    hfp half pint [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 38173, updated on 4-Jan-2025

    Summary

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    Official Symbol
    hfpprovided by FlyBase
    Official Full Name
    half pintprovided by FlyBase
    Primary source
    FLYBASE:FBgn0028577
    Locus tag
    Dmel_CG12085
    See related
    AllianceGenome:FB:FBgn0028577
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.408; CG12085; dFIR; Dmel\CG12085; DPUF68; Fir; Hfp; HFP; pUbsf; Puf60; PUF60; puf68; pUf68; pUF68; Puf68; PUF68
    Summary
    Predicted to enable RNA binding activity. Involved in several processes, including cystoblast division; mRNA splicing, via spliceosome; and regulation of alternative mRNA splicing, via spliceosome. Located in nucleus. Part of protein-containing complex. Is expressed in several structures, including adult head; central brain primordium; embryonic/larval midgut; embryonic/larval midgut primordium; and ganglia. Orthologous to human PUF60 (poly(U) binding splicing factor 60). [provided by Alliance of Genome Resources, Jan 2025]
    Orthologs
    all
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    Genomic context

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    See hfp in Genome Data Viewer
    Location:
    62A2-62A3; 3-0.5 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (1496853..1502590, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (1496853..1502590, complement)

    Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene stem cell tumor Neighboring gene ncRNA Neighboring gene antisense RNA:CR45812 Neighboring gene uncharacterized protein Neighboring gene small Cajal body-specific RNA : MeU2-48 Neighboring gene Puromycin sensitive aminopeptidase Neighboring gene cueball Neighboring gene Increased minichromosome loss 1 Neighboring gene Ionotropic receptor 62a

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Half pint couples transcription and splicing of eIF4E-1,2 gene during fly development. Reyes R, et al. Biochem Biophys Res Commun, 2008 Oct 3. PMID 18671938
    2. FUBP/KH domain proteins in transcription: Back to the future. Quinn LM. Transcription, 2017 May 27. PMID 28301294, Free PMC Article
    3. Defective Hfp-dependent transcriptional repression of dMYC is fundamental to tissue overgrowth in Drosophila XPB models. Lee JE, et al. Nat Commun, 2015 Jun 15. PMID 26074141, Free PMC Article
    4. Half pint/Puf68 is required for negative regulation of splicing by the SR splicing factor Transformer2. Wang S, et al. RNA Biol, 2013 Aug. PMID 23880637, Free PMC Article
    5. The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. Tamayo JV, et al. Cell Rep, 2017 Apr 4. PMID 28380354, Free PMC Article

    See all (96) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The data demonstrate that dMYC repression and dMYC-dependent overgrowth in the Hfp hypomorph is further impaired in the C-terminal Hay/XPB mutant background.
      Title: Defective Hfp-dependent transcriptional repression of dMYC is fundamental to tissue overgrowth in Drosophila XPB models.
    2. results indicate the Hfp/Puf68 protein is required for the negative regulation of splicing by Tra2
      Title: Half pint/Puf68 is required for negative regulation of splicing by the SR splicing factor Transformer2.
    3. regulation of dmyc requires interaction between the transcriptional repressor Hfp and the DNA helicase subunit of TFIIH, Haywire (Hay)
      Title: Hfp inhibits Drosophila myc transcription and cell growth in a TFIIH/Hay-dependent manner.
    4. These results suggest that the eIF4E-1,2 gene is regulated by Hfp through a mechanism linked to transcription control and 3' splice site selection, which determines the pattern and translation efficiency of eIF4E-1,2 mRNAs.
      Title: Half pint couples transcription and splicing of eIF4E-1,2 gene during fly development.
    5. Provides a critical molecular link between the the wingless pathway and dMyc-regulated cell growth and proliferation.
      Title: Drosophila Hfp negatively regulates dmyc and stg to inhibit cell proliferation.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in alternative mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cystoblast division IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA splice site recognition IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mRNA splice site recognition IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA splicing, via spliceosome IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of cell population proliferation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of alternative mRNA splicing, via spliceosome HMP PubMed 
    involved_in regulation of alternative mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of alternative mRNA splicing, via spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of catalytic step 2 spliceosome HDA PubMed 
    located_in nucleus IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of precatalytic spliceosome HDA PubMed 
    part_of protein-containing complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    half pint
    Names
    CG12085-PA
    CG12085-PB
    CG12085-PC
    CG12085-PD
    CG12085-PE
    CG12085-PF
    CG12085-PG
    CG12085-PH
    CG12085-PI
    CG12085-PJ
    half-pint
    hfp-PA
    hfp-PB
    hfp-PC
    hfp-PD
    hfp-PE
    hfp-PF
    hfp-PG
    hfp-PH
    hfp-PI
    hfp-PJ
    poly U binding factor 68kD
    polyU binding splicing factor

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_037436.4 Reference assembly

      Range
      1496853..1502590 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_080384.4 → NP_525123.2  half pint, isoform A [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q0E8J8, Q8T6B9, Q95S11, Q9U696, Q9W0E6, Q9W0E7
      Related
      FBpp0072593
      Conserved Domains (4) summary
      TIGR01645
      Location:10 → 637
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:130 → 205
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:227 → 303
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:536 → 636
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    2. NM_167880.3 → NP_728610.1  half pint, isoform B [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Related
      FBpp0072594
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    3. NM_001169848.2 → NP_001163319.1  half pint, isoform I [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    4. NM_001169845.2 → NP_001163316.1  half pint, isoform F [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    5. NM_001169849.2 → NP_001163320.1  half pint, isoform J [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    6. NM_001169847.2 → NP_001163318.1  half pint, isoform H [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    7. NM_167882.3 → NP_728612.1  half pint, isoform D [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Related
      FBpp0072592
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    8. NM_167881.3 → NP_728611.1  half pint, isoform C [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Related
      FBpp0072591
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    9. NM_001169846.2 → NP_001163317.1  half pint, isoform G [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins

    10. NM_001169844.2 → NP_001163315.1  half pint, isoform E [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A4V193
      Conserved Domains (4) summary
      TIGR01645
      Location:1 → 545
      half-pint; poly-U binding splicing factor, half-pint family
      cd12370
      Location:38 → 113
      RRM1_PUF60; RNA recognition motif 1 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12371
      Location:135 → 211
      RRM2_PUF60; RNA recognition motif 2 in (U)-binding-splicing factor PUF60 and similar proteins
      cd12648
      Location:444 → 544
      RRM3_UHM_PUF60; RNA recognition motif 3 in UHM domain of poly(U)-binding-splicing factor PUF60 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A4V193 A4V193
    Q8T6B9.2 Q8T6B9

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