RVB2 RuvB family ATP-dependent DNA helicase reptin [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RVB2
Official Full Name: RuvB family ATP-dependent DNA helicase reptin
Primary source: SGD:S000006156
Locus tag: YPL235W
See related: AllianceGenome:SGD:S000006156; FungiDB:YPL235W; VEuPathDB:YPL235W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TIH2; TIP48; TIP49B
Summary: Enables 5'-3' DNA helicase activity. Contributes to 3'-5' DNA helicase activity. Involved in several processes, including box C/D snoRNP assembly; rRNA processing; and regulation of transcription by RNA polymerase II. Located in nucleus. Part of Ino80 complex; R2TP complex; and Swr1 complex. Orthologous to human RUVBL2 (RuvB like AAA ATPase 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RVB2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (103232..104647)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation.
Title: Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation.
results demonstrate the chaperone-like potential of Rvb1/Rvb2 and suggest a model where binding of multiple clients such as Ino80 stimulates ATP-driven cycling between hexamers and dodecamers, providing iterative opportunities for correct subunit assembly.
Title: Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones.
Data show that the 14 different polypeptide containing chromatin-remodeling complex SWR1 contains a single heterohexameric Rvb1/Rvb2 ring that, together with the catalytic subunit Swr1, brackets two independently assembled multisubunit modules.
Title: Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1.
Existence of a novel role for Tel2, namely in transcription, possibly in cooperation with Rvb2 and involving the existence of physical interactions with the Med15/Gal11 Mediator subunit.
Title: Genetic and physical interactions between Tel2 and the Med15 Mediator subunit in Saccharomyces cerevisiae.
purified endogenous untagged Rvb1/Rvb2 complexes were found as single hexameric rings, similar to the complexes assembled in vitro from purified untagged components
Title: Alternative oligomeric states of the yeast Rvb1/Rvb2 complex induced by histidine tags.
Rvb2p, cooperatively binds single-stranded DNA as monomers. Binding stimulates a slow ATPase activity and supports a 3'- to 5' DNA unwinding activity that requires a 3'-protruding tail >or= 30 nucleotides.
Title: 3'- to 5' DNA unwinding by TIP49b proteins.
Pontin and reptin could acquire distinct structural states to regulate their broad functions as molecular motors and scaffolds for nucleic acids and proteins.
Title: Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes.
These results clearly establish the yeast Rvb1/2 complex as a heterohexameric ATP-dependent DNA helicase and highlight the possible roles played by the Rvb proteins within multiprotein complexes.
Title: Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex.
Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA; both proteins also interact with chromatin and play a role in recombination and repair by chromatin remodelling.
Title: Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-3' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in box C/D snoRNP assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in box C/D snoRNP assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IPI Inferred from Physical Interaction more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Ino80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ino80 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of NuA4 histone acetyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of R2TP complex	IBA Inferred from Biological aspect of Ancestor more info
part_of R2TP complex	IDA Inferred from Direct Assay more info	PubMed
part_of R2TP complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Swr1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Swr1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Swr1 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of TTT Hsp90 cochaperone complex	HDA more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RuvB family ATP-dependent DNA helicase reptin

NP_015089.1

EC 3.6.4.12

ATP-dependent DNA helicase, also known as reptin; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, the R2TP complex (Rvb1-Rvb2-Tah1-Pih1) and the TTT Hsp90 cochaperone complex that is involved in chromatin remodeling and telomeric chromatin regulation; involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly; member of the AAA+ and RuvB protein families, similar to Rvb1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

103232..104647

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184049.1 → NP_015089.1 TPA: RuvB family ATP-dependent DNA helicase reptin [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015089.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3D5, Q12464

UniProtKB/TrEMBL

A6ZW20, B3LKJ6, B5VSU7, C7GU74, C8ZIE4, G2WNV0, N1NWJ1

Conserved Domains (1) summary

COG1224
Location:4 → 448

TIP49; DNA helicase TIP49, TBP-interacting protein [Transcription]