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    PRI2 DNA primase subunit PRI2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853821, updated on 9-Dec-2024

    Summary

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    Official Symbol
    PRI2
    Official Full Name
    DNA primase subunit PRI2
    Primary source
    SGD:S000001528
    Locus tag
    YKL045W
    See related
    AllianceGenome:SGD:S000001528; FungiDB:YKL045W; VEuPathDB:YKL045W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Contributes to DNA primase activity and single-stranded DNA binding activity. Involved in DNA replication, synthesis of primer and double-strand break repair. Located in nuclear envelope. Part of alpha DNA polymerase:primase complex. Orthologous to human PRIM2 (DNA primase subunit 2). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See PRI2 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (353493..355079)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Anr2p Neighboring gene putative mannan endo-1,6-alpha-mannosidase Neighboring gene Mmo1p Neighboring gene Phd1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae. Foiani M, et al. Mol Cell Biol, 1989 Jul. PMID 2528682, Free PMC Article
    2. A DNA primase from yeast. Purification and partial characterization. Jazwinski SM, et al. J Biol Chem, 1985 Apr 25. PMID 3988742
    3. An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis. Klinge S, et al. Nat Struct Mol Biol, 2007 Sep. PMID 17704817, Free PMC Article
    4. Yeast require redox switching in DNA primase. O'Brien E, et al. Proc Natl Acad Sci U S A, 2018 Dec 26. PMID 30541886, Free PMC Article
    5. Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase. Sauguet L, et al. PLoS One, 2010 Apr 9. PMID 20404922, Free PMC Article

    See all (61) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data indicate that a defective DNA polymerase alpha (Polalpha)/DNA primase/chromatin-binding protein Ctf4 complex causes unscheduled strand annealing and fork reversal.
      Title: Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Polα/Primase/Ctf4 Complex.
    2. crystal structure of the PriL- C-terminus domain at 1.55 A resolution reports the first crystallographic model of the conserved Fe-S domain
      Title: Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to DNA primase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to DNA primase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    contributes_to DNA primase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication, synthesis of primer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication, synthesis of primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA-templated DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of alpha DNA polymerase:primase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of alpha DNA polymerase:primase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA primase subunit PRI2
    NP_012879.1
    • Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      353493..355079
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179611.1 → NP_012879.1  TPA: DNA primase subunit PRI2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012879.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXP2, P20457
      UniProtKB/TrEMBL
      A6ZZS1, G2WHY7, N1P2E5
      Conserved Domains (1) summary
      cd07322
      Location:61 → 507
      PriL_PriS_Eukaryotic; Eukaryotic core primase: Large subunit, PriL
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P20457.1

    Gene LinkOut

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