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    arp3 ARP2/3 actin-organizing complex actin-related protein subunit Arp3 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2543233, updated on 18-Sep-2024

    Summary

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    Official Symbol
    arp3
    Official Full Name
    ARP2/3 actin-organizing complex actin-related protein subunit Arp3
    Primary source
    PomBase:SPAC630.03
    Locus tag
    SPOM_SPAC630.03
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    act2
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    Genomic context

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    See arp3 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    5
    Sequence:
    Chromosome: I; NC_003424.3 (350433..352296)

    Chromosome I - NC_003424.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene rRNA-processing protein Neighboring gene GATOR2-SEACAT complex ubiquitin-protein ligase E3 subunit Sea4 Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene uncharacterized protein Neighboring gene GTPase-activating protein Gyp7

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003424.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction. Chou SZ, et al. Proc Natl Acad Sci U S A, 2022 Dec 6. PMID 36442092, Free PMC Article
    2. Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state. Shaaban M, et al. Nat Struct Mol Biol, 2020 Nov. PMID 32839613, Free PMC Article
    3. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches. Pandit NG, et al. Proc Natl Acad Sci U S A, 2020 Jun 16. PMID 32461373, Free PMC Article
    4. Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers. Burke TA, et al. Curr Biol, 2014 Mar 3. PMID 24560576, Free PMC Article
    5. Dip1 Co-opts Features of Branching Nucleation to Create Linear Actin Filaments that Activate WASP-Bound Arp2/3 Complex. Balzer CJ, et al. Curr Biol, 2018 Dec 3. PMID 30471998, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
      Title: Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
    2. Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
      Title: Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
    3. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
      Title: Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
    4. WASp-VCA binding to Arp2/3 protein complex causes a conformational change that favors binding to the side of an actin filament, which allows further changes required to nucleate a daughter filament.
      Title: Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.
    5. ARP3 role in the actin filament nucleation.ATP binding destabilizes this interaction via an allosteric link between the Arp3 nucleotide cleft and the hydrophobic groove, thereby promoting the short-pitch conformation.
      Title: Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.
    6. Arp2/3 complex participates in regulating formin F-actin networks.
      Title: Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers.
    7. Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex
      Title: Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.
    8. Cofilin rapidly dissociates Arp2/3 complex and branches by direct competition for binding sites on the actin filament and by propagation of structural changes in the actin filament that reduce affinity for Arp2/3 complex.
      Title: Cofilin dissociates Arp2/3 complex and branches from actin filaments.
    9. Truncation of the N terminus of ARPC5, the smallest subunit in the complex, increased the yield of Arp2Delta Arp2/3 complex during purification but did not compromise nucleation activity of the full Arp2/3 complex.
      Title: Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables actin filament binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables actin filament binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Arp2/3 complex-mediated actin nucleation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in Arp2/3 complex-mediated actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in actin cortical patch organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin filament branching EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in actin filament polymerization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endocytosis IC
    Inferred by Curator
    more info
    		  
    Component Evidence Code Pubs
    part_of Arp2/3 protein complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of Arp2/3 protein complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in actin cortical patch IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in actin cortical patch IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cell cortex of cell tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cell division site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm HDA PubMed 
    is_active_in cytosol HDA PubMed 

    General protein information

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    Preferred Names
    ARP2/3 actin-organizing complex actin-related protein subunit Arp3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003424.3 Reference assembly

      Range
      350433..352296
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001018298.3 → NP_592898.1  ARP2/3 actin-organizing complex actin-related protein subunit Arp3 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_592898.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P32390
      Conserved Domains (1) summary
      PTZ00280
      Location:8 → 425
      PTZ00280; Actin-related protein 3; Provisional
    Nucleotide Protein
    Heading Accession and Version

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