MAD3 Mad3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MAD3
Official Full Name: Mad3p
Primary source: SGD:S000003550
Locus tag: YJL013C
See related: AllianceGenome:SGD:S000003550; FungiDB:YJL013C; VEuPathDB:YJL013C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in distributive segregation; negative regulation of cell cycle phase transition; and positive regulation of protein autoubiquitination. Part of mitotic checkpoint complex. Human ortholog(s) of this gene implicated in colorectal cancer; mosaic variegated aneuploidy syndrome 1; and primary autosomal recessive microcephaly. Orthologous to human BUB1 (BUB1 mitotic checkpoint serine/threonine kinase) and BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MAD3 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (409493..411040, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Phosphatase PP1 Promotes Mitotic Slippage through Mad3 Dephosphorylation.
Title: The Phosphatase PP1 Promotes Mitotic Slippage through Mad3 Dephosphorylation.
The Bub1 TPR domain is both necessary and sufficient to bind and recruit Mad3.
Title: The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.
our results are consistent with a role of Mad3 as the main interactor of Mad2 via the dimerization surface. Mad3 being required to stabilize the Cdc20:C-Mad2 complex implies that the complex by itself is unstable.
Title: Role of the Mad2 dimerization interface in the spindle assembly checkpoint independent of kinetochores.
Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Title: Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.
data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation
Title: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.
Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
Title: Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.
model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction
Title: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.
In vivo Mad3 phosphorylation depends on the Polo kinase Cdc5 and, to a minor extent, the Aurora B kinase Ipl1.
Title: Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in distributive segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic sister chromatid cohesion, centromeric	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA integrity checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein autoubiquitination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of mitotic checkpoint complex	IDA Inferred from Direct Assay more info	PubMed
part_of mitotic checkpoint complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of mitotic checkpoint complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Mad3p

NP_012521.3

Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

409493..411040 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181447.3 → NP_012521.3 TPA: Mad3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012521.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWG4, P47074

UniProtKB/TrEMBL

G2WGZ8, N1P398

Conserved Domains (2) summary

smart00777
Location:57 → 188

Mad3_BUB1_I; Mad3/BUB1 hoMad3/BUB1 homology region 1

pfam08171
Location:330 → 397

Mad3_BUB1_II; Mad3/BUB1 homology region 2