rad50 DNA repair protein Rad50 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: rad50
Official Full Name: DNA repair protein Rad50
Primary source: PomBase:SPAC1556.01c
Locus tag: SPOM_SPAC1556.01C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: SPAP4C9.01c
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Genomic context

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See rad50 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 5

Sequence:: Chromosome: I; NC_003424.3 (3791753..3795998, complement)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N-terminus region of Drp1, a Rint1 family protein is essential for cell survival and its interaction with Rad50 protein in fission yeast S.pombe.
Title: The N-terminus region of Drp1, a Rint1 family protein is essential for cell survival and its interaction with Rad50 protein in fission yeast S.pombe.
a mre11 mutant defective in endonucleolytic activity, the same mutant lacking Ctp1, or the triple mutant also lacking the putative hairpin nuclease Pso2 showed wild-type levels of repair. Thus, MRN (Mre11-Rad50-Nbs1)may act to recruit the hairpin opening activity that allows subsequent repair
Title: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe.
Mre11-Rad50-Nbs1 participates in checkpoint-dependent replication slowing through negative regulation of recombination.
Title: The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.
Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
Study finds that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN.
Title: Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.
The Mre11-Rad50-Nbs1 S-phase checkpoint role is separate from its Ctp1- and resection-dependent role in double-strand break repair.
Title: The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling.
This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32(Mre11)) nuclease activity is required for the removal of Rec12(Spo11).
Title: Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions.
The conserved Mre11/Rad50/Nbs1 (MRN) complex is likely directly involved in processing palindromes through the homologous recombination pathway of DNA repair.
Title: A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombe.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	ISM Inferred from Sequence Model more info
enables ATP hydrolysis activity	NAS Non-traceable Author Statement more info
enables DNA end binding	EXP Inferred from Experiment more info	PubMed
enables DNA helicase activity	ISO Inferred from Sequence Orthology more info
enables G-quadruplex DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization involved in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair involved in meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IC Inferred by Curator more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gene conversion at mating-type locus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination-dependent replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stalled replication fork localization to nuclear periphery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via telomerase	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Mre11 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Mre11 complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in chromatin	IC Inferred by Curator more info
is_active_in condensed nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA repair protein Rad50

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

3791753..3795998 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001356129.2 → NP_001342968.1 DNA repair protein Rad50 [Schizosaccharomyces pombe]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

Q9P3T5, Q9UTJ8

Conserved Domains (1) summary

cl25732
Location:4 → 1282

SMC_N; RecF/RecN/SMC N terminal domain