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    pkl1 kinesin-14 family minus-end directed microtubule motor Pkl1 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2542655, updated on 18-Sep-2024

    Summary

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    Official Symbol
    pkl1
    Official Full Name
    kinesin-14 family minus-end directed microtubule motor Pkl1
    Primary source
    PomBase:SPAC3A11.14c
    Locus tag
    SPOM_SPAC3A11.14C
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    klp1; SPAC3H5.03c
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    Genomic context

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    See pkl1 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    4
    Sequence:
    Chromosome: I; NC_003424.3 (3438890..3442117)

    Chromosome I - NC_003424.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 40S ribosomal protein uS11 Neighboring gene U5 snRNP-associated protein Aar2 Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene tRNA-Arg Neighboring gene prefoldin subunit 6

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003424.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1. Furuta K, et al. J Biol Chem, 2008 Dec 26. PMID 18984586, Free PMC Article
    2. Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function. Pidoux AL, et al. Mol Biol Cell, 1996 Oct. PMID 8898367, Free PMC Article
    3. A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p. Paluh JL, et al. Mol Biol Cell, 2000 Apr. PMID 10749926, Free PMC Article
    4. Distinct Kinesin-14 mitotic mechanisms in spindle bipolarity. Simeonov DR, et al. Cell Cycle, 2009 Nov 1. PMID 19838064
    5. Kinesin-14 family proteins and microtubule dynamics define S. pombe mitotic and meiotic spindle assembly, and elongation. Loncar A, et al. J Cell Sci, 2020 Jun 8. PMID 32327557, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. although deletion of either pkl1 or klp2 rescues temperature-sensitive cut7 mutants, deletion of only pkl1 can bypass the lethality caused by cut7 deletion
      Title: Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe.
    2. Loss of kinesin-14 Pkl1, leads to kinesin-5 Cut7-dependent aberrant long spindle microtubule minus-end protrusions that push the properly segregated chromosomes to the site of cell division, resulting in chromosome cut at cytokinesis and aneuploidy.
      Title: Loss of kinesin-14 results in aneuploidy via kinesin-5-dependent microtubule protrusions leading to chromosome cut.
    3. spindle anchoring through Msd1-Wdr8-Pkl1 is crucial for balancing the Cut7/kinesin-5-mediated outward force at the spindle pole body.
      Title: The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.
    4. analysis of the in vitro motile properties of fission yeast kinesin-14 Pkl1
      Title: Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.
    5. Ultrastructural analysis of mutant mitotic spindles suggests that Pkl1p contributes to error-free biorientation by promoting normal spindle pole organization, whereas dynein helps to anchor a focused bundle of spindle microtubules at the pole.
      Title: Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables microtubule motor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule motor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables minus-end-directed microtubule motor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables minus-end-directed microtubule motor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in meiotic spindle assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in microtubule anchoring at mitotic spindle pole body IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in microtubule bundle formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in microtubule-based movement IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic sister chromatid segregation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic spindle assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic spindle assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic spindle organization EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in protein transport along microtubule to mitotic spindle pole body IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of MWP complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of kinesin complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in meiotic spindle pole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in meiotic spindle pole body EXP
    Inferred from Experiment
    more info
    		 PubMed 
    is_active_in microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in microtubule organizing center IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in mitotic spindle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in mitotic spindle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle pole body HDA PubMed 
    is_active_in mitotic spindle pole body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    kinesin-14 family minus-end directed microtubule motor Pkl1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003424.3 Reference assembly

      Range
      3438890..3442117
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001019613.3 → NP_594189.1  kinesin-14 family minus-end directed microtubule motor Pkl1 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_594189.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      O42669, Q92376
      Conserved Domains (3) summary
      smart00129
      Location:479 → 829
      KISc; Kinesin motor, catalytic domain. ATPase
      cd01366
      Location:477 → 823
      KISc_C_terminal; Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins
      pfam16796
      Location:458 → 486
      Microtub_bd; Microtubule binding
    Nucleotide Protein
    Heading Accession and Version

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