CIT2 citrate (Si)-synthase CIT2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CIT2
Official Full Name: citrate (Si)-synthase CIT2
Primary source: SGD:S000000598
Locus tag: YCR005C
See related: AllianceGenome:SGD:S000000598; FungiDB:YCR005C; VEuPathDB:YCR005C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables citrate (Si)-synthase activity. Involved in citrate metabolic process. Located in peroxisome. Human ortholog(s) of this gene implicated in dilated cardiomyopathy and hypertrophic cardiomyopathy. Orthologous to human CS (citrate synthase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CIT2 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (120946..122328, complement)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In S. cerevisiae hydroxycitric acid antagonizes chronological aging and apoptosis regardless of citrate lyase.
Title: In S. cerevisiae hydroxycitric acid antagonizes chronological aging and apoptosis regardless of citrate lyase.
study tested the ability of all nine S. cerevisiae basic helix-loop-helix (bHLH) proteins to regulate the CIT2 gene
Title: Multiple bHLH proteins regulate CIT2 expression in Saccharomyces cerevisiae.
Overexpression of CIT2 causes a twofold overexpression of fumarase and an alteration in fumarase distribution.
Title: Dual localization of fumarase is dependent on the integrity of the glyoxylate shunt.
overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Delta
Title: Regulation of respiratory growth by Ras: the glyoxylate cycle mutant, cit2Delta, is suppressed by RAS2.
The minimum ranges of the cryptic signals for mitochondrial and peroxisomal targeting of Cit2 were shown to consist of the first 15- and 10-amino acid N-terminal segments, respectively
Title: Mutational and functional analysis of the cryptic N-terminal targeting signal for both mitochondria and peroxisomes in yeast peroxisomal citrate synthase Cit2p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables citrate (Si)-synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables citrate (Si)-synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables citrate (Si)-synthase activity	IEA Inferred from Electronic Annotation more info
enables citrate (Si)-synthase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables citrate synthase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in citrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in citrate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in tricarboxylic acid cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in tricarboxylic acid cycle	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: citrate (Si)-synthase CIT2

NP_009931.1

EC 2.3.3.16

Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

120946..122328 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178718.1 → NP_009931.1 TPA: citrate (Si)-synthase CIT2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009931.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VR15, P08679

UniProtKB/TrEMBL

A6ZTI7, B3LU77, B5VEV3, C7GQL8, C8Z490, G2WA23, N1P997

Conserved Domains (1) summary

cl00416
Location:26 → 452

CS_ACL-C_CCL; Citrate synthase (CS), citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CS catalyzes the condensation of acetyl coenzyme A ...