tut-1 Cytoplasmic tRNA 2-thiolation protein 1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: tut-1
Official Full Name: Cytoplasmic tRNA 2-thiolation protein 1
Primary source: WormBase:WBGene00017928
Locus tag: CELE_F29C4.6
See related: AllianceGenome:WB:WBGene00017928
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables sulfurtransferase activity and tRNA binding activity. Involved in several processes, including oocyte development; tRNA modification; and vulval development. Predicted to be located in cytosol. Predicted to be part of cytosolic tRNA wobble base thiouridylase complex. Orthologous to human CTU1 (cytosolic thiouridylase subunit 1). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See tut-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 3

Sequence:: Chromosome: IV; NC_003282.8 (119269..121707, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

inactivation of the complex leads to loss of thiolation on tRNAs and to a thermosensitive decrease of viability associated with marked ploidy abnormalities and aberrant development
Title: The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F29C4.6 (e-PCR)
- UniSTS:314159

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables sulfurtransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables tRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables tRNA binding	IEA Inferred from Electronic Annotation more info
enables tRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in embryonic morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oocyte development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein urmylation	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in tRNA thio-modification	IEA Inferred from Electronic Annotation more info
involved_in tRNA thio-modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA thio-modification	ISS Inferred from Sequence or Structural Similarity more info
involved_in tRNA wobble position uridine thiolation	IBA Inferred from Biological aspect of Ancestor more info
involved_in tRNA wobble uridine modification	IEA Inferred from Electronic Annotation more info
involved_in tRNA wobble uridine modification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in tRNA wobble uridine modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA wobble uridine modification	ISS Inferred from Sequence or Structural Similarity more info
involved_in translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vulval development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
part_of cytosolic tRNA wobble base thiouridylase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Cytoplasmic tRNA 2-thiolation protein 1

NP_499865.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

119269..121707 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_067464.5 → NP_499865.1 Cytoplasmic tRNA 2-thiolation protein 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499865.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76365

Conserved Domains (3) summary

TIGR00269
Location:209 → 310

TIGR00269; TIGR00269 family protein

COG0037
Location:35 → 311

TilS; tRNA(Ile)-lysidine synthase TilS/MesJ [Translation, ribosomal structure and biogenesis]

cd01993
Location:59 → 245

Alpha_ANH_like_II; This is a subfamily of Adenine nucleotide alpha hydrolases superfamily.Adeninosine nucleotide alpha hydrolases superfamily includes N type ATP PPases and ATP sulphurylases. It forms a apha/beta/apha fold which binds to Adenosine group. This subfamily ...