gpa-16 Guanine nucleotide-binding protein alpha-16 subunit [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: gpa-16
Official Full Name: Guanine nucleotide-binding protein alpha-16 subunit
Primary source: WormBase:WBGene00001678
Locus tag: CELE_Y95B8A.5
See related: AllianceGenome:WB:WBGene00001678
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable several functions, including G-protein beta/gamma-subunit complex binding activity; GTPase activity; and guanyl ribonucleotide binding activity. Involved in embryonic axis specification and establishment of mitotic spindle orientation. Located in cell cortex. Is expressed in several structures, including AB; C; D; neurons; and pharynx. Human ortholog(s) of this gene implicated in achromatopsia 4; color blindness; congenital stationary night blindness 1G; congenital stationary night blindness autosomal dominant 3; and night blindness. Orthologous to several human genes including GNAI1 (G protein subunit alpha i1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See gpa-16 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 8

Sequence:: Chromosome: I; NC_003279.8 (897286..907370)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results suggest that Galpha signaling and LET-99 control centration by regulating polarized actomyosin contraction.
Title: Control of nuclear centration in the C. elegans zygote by receptor-independent Galpha signaling and myosin II.
requirement toward GPA-16 is distinct from the known function of RIC-8 in enabling interaction between Galpha proteins and GPR-1/2
Title: Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division.
findings show that the GoLoco-containing proteins GPR-1 and GPR-2, as well as the Galpha subunits GOA-1 and GPA-16, were essential for generation of proper pulling forces in asymmetric spindle positioning
Title: Translation of polarity cues into asymmetric spindle positioning in Caenorhabditis elegans embryos.
Results show that the spn-1 gene encodes the Galpha protein GPA-16, which appears to be required for centrosomal association of a Gbeta protein.
Title: Embryonic handedness choice in C. elegans involves the Galpha protein GPA-16.
Data show that the asymmetry of GPR-1/2 (which act with GOA-1 and GPA-16) localization depends on PAR-3 and its downstream intermediate LET-99, as well as on the MES-1/SRC-1 signaling pathway.
Title: LET-99 opposes Galpha/GPR signaling to generate asymmetry for spindle positioning in response to PAR and MES-1/SRC-1 signaling.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables GDP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables guanyl nucleotide binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in embryonic axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Preferred Names: Guanine nucleotide-binding protein alpha-16 subunit

NP_001293186.1

Confirmed by transcript evidence

NP_001293187.1

Confirmed by transcript evidence

NP_490790.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

897286..907370

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_058389.4 → NP_490790.1 Guanine nucleotide-binding protein alpha-16 subunit [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_490790.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9N2V6

Conserved Domains (2) summary

cd00066
Location:34 → 351

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)

smart00275
Location:13 → 355

G_alpha; G protein alpha subunit
NM_001306257.3 → NP_001293186.1 Guanine nucleotide-binding protein alpha-16 subunit [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293186.1

Status: REVIEWED

UniProtKB/TrEMBL

V6CK09

Conserved Domains (1) summary

cl38936
Location:1 → 120

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001306258.3 → NP_001293187.1 Guanine nucleotide-binding protein alpha-16 subunit [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293187.1

Status: REVIEWED

UniProtKB/TrEMBL

V6CLF2

Conserved Domains (1) summary

cl38936
Location:1 → 109

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases