insc inscuteable [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: inscprovided by FlyBase
Official Full Name: inscuteableprovided by FlyBase
Primary source: FLYBASE:FBgn0011674
Locus tag: Dmel_CG11312
See related: AllianceGenome:FB:FBgn0011674
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 25/17; CG11312; Dmel\CG11312; fam; Ins; Insc; INSC; l(2)05475; l(2)k12405; nem
Summary: Enables cytoskeletal anchor activity. Involved in several processes, including Malpighian tubule tip cell differentiation; apical protein localization; and nervous system development. Located in apical cortex. Part of raps-insc complex. Is expressed in several structures, including anlage in statu nascendi; gut section; head epidermis primordium; midgut primordium; and somatic precursor cell. Used to study Charcot-Marie-Tooth disease type 2. Orthologous to human INSC (INSC spindle orientation adaptor protein). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 57B3-57B3; 2-92 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (20821262..20836028, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (16708767..16723533, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A missense mutation in human INSC causes peripheral neuropathy.
Title: A missense mutation in human INSC causes peripheral neuropathy.
LGN in complex with the asymmetric domain of Inscuteable, which reveals a tetrameric arrangement of intertwined molecules.
Title: Insc:LGN tetramers promote asymmetric divisions of mammary stem cells.
Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain
Title: Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain.
Insc-Pins complex requires Galphai for partial activity and that the complex specifically recruits Dlg but not Mud.
Title: Inscuteable regulates the Pins-Mud spindle orientation pathway.
The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
Title: The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
Inscuteable and NuMA [Mud] proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions.
Title: Inscuteable and NuMA proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions.
three neuroblast lineages (NB1-1, NB4-2, and NB7-1) require insc function for sibling cell fate specification in cells born from early ganglion mother cells (GMCs) whereas insc is not required in cells born from later GMCs of the same lineages
Title: On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages.
we show that Inscuteable, a multiadapter protein required for asymmetric cell division, participates in the formation of specific heart cells to distinguish between a myogenic (cardiomyoblast) and a nonmyogenic (pericardial cell) fate.
Title: Cell fate decisions in the Drosophila dorsal vessel depend on the multiadapter protein inscuteable.
Insc is required for accurate control of metaphase spindle length, division orientation, and asymmetric cell division.
Title: Inscuteable mRNA localization is dynein-dependent and regulates apicobasal polarity and spindle length in Drosophila neuroblasts.
Results show that many identified neuroblast lineages do not require inscuteable for normal morphological development.
Title: Inscuteable-independent apicobasally oriented asymmetric divisions in the Drosophila embryonic CNS.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

Dm0306 (e-PCR)
- UniSTS:73584

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables cytoskeletal anchor activity	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal anchor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Malpighian tubule tip cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical protein localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in apical protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical protein localization	IPI Inferred from Physical Interaction more info	PubMed
involved_in asymmetric cell division	IEA Inferred from Electronic Annotation more info
involved_in asymmetric neuroblast division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in neuroblast fate determination	IDA Inferred from Direct Assay more info	PubMed
involved_in peripheral nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of asymmetric cell division	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory organ development	TAS Traceable Author Statement more info	PubMed
involved_in somatic muscle development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in apical cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of raps-insc complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: inscuteable

Names: CG11312-PA; fata morgana; insc-PA; inscrutable; inscutable; not enough muscles

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

20821262..20836028 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057676.4 → NP_477024.1 inscuteable [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477024.1

Status: REVIEWED

UniProtKB/TrEMBL

Q24367, Q9W2R4

Related

FBpp0071490

Conserved Domains (1) summary

COG5253
Location:62 → 142

MSS4; Phosphatidylinositol-4-phosphate 5-kinase [Signal transduction mechanisms]