AGO1 Argonaute-1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: AGO1provided by FlyBase
Official Full Name: Argonaute-1provided by FlyBase
Primary source: FLYBASE:FBgn0262739
Locus tag: Dmel_CG6671
See related: AllianceGenome:FB:FBgn0262739
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Ago; ago-1; Ago-1; AGO-1; ago1; Ago1; ago1-1; Agp1; anon-WO0257455.29; argonaute1; CG6671; dAgo1; dAGO1; Dm Ago1; dmAgo1; DmAgo1; Dmel\CG6671; l(2)04845; l(2)4845; l(2)k00208; l(2)k08121; MRE20
Summary: Enables RNA binding activity; endoribonuclease activity, cleaving miRNA-paired mRNA; and enzyme binding activity. Involved in several processes, including female germ-line stem cell population maintenance; germarium-derived egg chamber formation; and negative regulation of gene expression. Located in P-body; nucleus; and perinuclear region of cytoplasm. Part of RISC complex and RISC-loading complex. Is expressed in several structures, including adult head; antennal disc; embryonic/larval proventriculus; embryonic/larval salivary gland; and germline cell. Human ortholog(s) of this gene implicated in alcohol dependence and glaucoma. Orthologous to several human genes including AGO2 (argonaute RISC catalytic component 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 50C9-50C17; 2-68 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (13943387..13958089, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (9830892..9845594, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999.
Title: Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999.
Age-Related Argonaute Loading of Ribosomal RNA Fragments.
Title: Age-Related Argonaute Loading of Ribosomal RNA Fragments.
Transcriptional repression of Myc underlies the tumour suppressor function of AGO1 in Drosophila.
Title: Transcriptional repression of Myc underlies the tumour suppressor function of AGO1 in Drosophila.
VCP Machinery Mediates Autophagic Degradation of Empty Argonaute.
Title: VCP Machinery Mediates Autophagic Degradation of Empty Argonaute.
We chose Drosophila as a model system to take advantage of the presence of two biochemically distinct Argonautes, AGO1 and AGO2. Our results revealed AGO2 loading to be strongly favored by G-rich sequences. In contrast, AGO1 showed an enrichment of the 'GAC' motif in loaded species.
Title: Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes.
Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
Title: Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
findings propose that Ago-1 acts as a key regulator in controlling cell death, tumor regression and stress response in metazoan providing a constructive bridge between RNAi machinery and cell death
Title: Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it.
Results suggest that Wisp interacts with Ago1 and induces miRNA adenylation and facilitates its downregulation.
Title: Adenylation of maternally inherited microRNAs by Wispy.
Results have established a novel role of Ago-1 as a regulator of the cell cycle.
Title: Argonaute-1 functions as a mitotic regulator by controlling Cyclin B during Drosophila early embryogenesis.
AGO1 protein level is reduced in the absence of miRNA biogenesis & accumulates on depletion of GW182. Ectopic transcription of miRNAs within in vivo clones & genetic interference with the ubiquitin-proteasome system induced accumulation of AGO1.
Title: Homeostatic control of Argonaute stability by microRNA availability.

Submit: New GeneRIF Correction See all GeneRIFs (32)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables endoribonuclease activity, cleaving miRNA-paired mRNA	IDA Inferred from Direct Assay more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in female germ-line stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived female germ-line cyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in habituation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in miRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of olfactory learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in segment polarity determination	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	IPI Inferred from Physical Interaction more info	PubMed
part_of RISC-loading complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Argonaute-1

Names: AGO1-PA; AGO1-PB; AGO1-PC; AGO1-PD; Argonaute1; CG6671-PA; CG6671-PB; CG6671-PC; CG6671-PD; argonaute; argonaute 1; argonaute protein 1; argonaute-1; mRNA-like ncRNA in embryogenesis 20

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

13943387..13958089 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001259385.1 → NP_001246314.1 Argonaute-1, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246314.1

Status: REVIEWED

UniProtKB/TrEMBL

Q27IR0, Q32KD4

Conserved Domains (4) summary

cd02846
Location:336 → 456

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:502 → 942

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:285 → 335

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:146 → 275

ArgoN; N-terminal domain of argonaute
NM_166021.2 → NP_725342.1 Argonaute-1, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725342.1

Status: REVIEWED

UniProtKB/TrEMBL

Q27IR0, Q32KD4

Related

FBpp0086738

Conserved Domains (4) summary

cd02846
Location:336 → 456

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:502 → 942

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:285 → 335

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:146 → 275

ArgoN; N-terminal domain of argonaute
NM_079010.4 → NP_523734.1 Argonaute-1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523734.1

Status: REVIEWED

UniProtKB/TrEMBL

Q27IR0, Q7KY08

Related

FBpp0086740

Conserved Domains (4) summary

cd02846
Location:302 → 422

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:468 → 908

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:251 → 301

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:112 → 241

ArgoN; N-terminal domain of argonaute
NM_166020.2 → NP_725341.1 Argonaute-1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725341.1

Status: REVIEWED

UniProtKB/TrEMBL

Q27IR0, Q32KD4

Related

FBpp0086739

Conserved Domains (4) summary

cd02846
Location:336 → 456

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:502 → 942

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:285 → 335

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:146 → 275

ArgoN; N-terminal domain of argonaute