GTR1 Rag GTPase GTR1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GTR1
Official Full Name: Rag GTPase GTR1
Primary source: SGD:S000004590
Locus tag: YML121W
See related: AllianceGenome:SGD:S000004590; FungiDB:YML121W; VEuPathDB:YML121W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activity. Involved in several processes, including DNA-templated transcription; negative regulation of metabolic process; and positive regulation of TORC1 signaling. Acts upstream of or within cellular response to oxidative stress. Located in fungal-type vacuole membrane; late endosome membrane; and nucleus. Part of Gtr1-Gtr2 GTPase complex and Ragulator complex. Orthologous to several human genes including RRAGB (Ras related GTP binding B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GTR1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (26930..27862)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Involvement of Gtr1p in the oxidative stress response in yeast Saccharomyces cerevisiae.
Title: Involvement of Gtr1p in the oxidative stress response in yeast Saccharomyces cerevisiae.
Ivy1 colocalizes with Gtr1 and Gtr2, a heterodimer of small GTPases that are part of the EGO complex.
Title: Ivy1 is a negative regulator of Gtr-dependent TORC1 activation.
the early-endosome recycling route and its control though the Vam6>Gtr1/Gtr2>Ltv1 pathway plays a physiological role in regulating the abundance of amino acid transporters at the cell surface
Title: Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases.
Data indicate that the metabolic input acts independently of small GTPases Gtr1 and Gtr2/Rag in TOR complex 1 (TORC1) activation.
Title: Nitrogen source activates TOR (target of rapamycin) complex 1 via glutamine and independently of Gtr/Rag proteins.
Study demonstrates V-ATPase interacts with two distinct GTPases, Arf1 and Gtr1, which are required for Ras and TORC1 activation, respectively.
Title: Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity.
The altered GTPase activity of gtr1 protein was shown associated conformational changes as compared to the wild type protein and the cysteine-less mutant.
Title: The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae.
Crystal structure of the Gtr1p(GTP)-Gtr2p(GDP) protein complex reveals large structural rearrangements triggered by GTP-to-GDP conversion.
Title: Crystal structure of the Gtr1p(GTP)-Gtr2p(GDP) protein complex reveals large structural rearrangements triggered by GTP-to-GDP conversion.
Data present the crystal structure of the Gtr1p-Gtr2p complex, the Rag homologs from Saccharomyces cerevisiae, at 2.8 A resolution.
Title: Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation.
Vam6 controls TORC1(Tco89) function by activating the Gtr1 subunit of the EGO complex.
Title: The Vam6 GEF controls TORC1 by activating the EGO complex.
Findings indicate that the formation of heterodimers by Gtr1p differs between Gtr2p and Ego1p.
Title: Gtr1p differentially associates with Gtr2p and Ego1p.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to amino acid stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrogen starvation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of autophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphate ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of TORC1 signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of TORC1 signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of TORC1 signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to vacuolar membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase I	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Gtr1-Gtr2 GTPase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Gtr1-Gtr2 GTPase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Ragulator complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rag GTPase GTR1

NP_013585.1

Subunit of TORC1-stimulating GTPase and EGO/GSE complex; subunit of Gtr1-Gtr2 GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p; involved in phosphate transport, telomeric chromatin silencing and oxidative stress response; activated by Iml1p (GAP) subunit of SEACIT complex; targeted to vacuole via AP-3 pathway; similar to human RagA and RagB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

26930..27862

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182484.1 → NP_013585.1 TPA: Rag GTPase GTR1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013585.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0G3, Q00582

UniProtKB/TrEMBL

A6ZLU3, B3LLD8, B5VP09, C7GVA3, C8ZE81, G2WJT5, N1P024

Conserved Domains (1) summary

cd11384
Location:8 → 304

RagA_like; Rag GTPase, subfamily of Ras-related GTPases, includes Ras-related GTP-binding proteins A and B