TIM50 protein translocase subunit TIM50 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TIM50
Official Full Name: protein translocase subunit TIM50
Primary source: SGD:S000005984
Locus tag: YPL063W
See related: AllianceGenome:SGD:S000005984; FungiDB:YPL063W; VEuPathDB:YPL063W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mitochondrion targeting sequence binding activity. Contributes to protein transmembrane transporter activity. Involved in protein import into mitochondrial matrix and regulation of mitochondrial membrane permeability. Located in mitochondrion. Part of TIM23 mitochondrial import inner membrane translocase complex. Human ortholog(s) of this gene implicated in 3-methylglutaconic aciduria type 9. Orthologous to human TIMM50 (translocase of inner mitochondrial membrane 50). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TIM50 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (429939..431369)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23.
Title: A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23.
The presequence translocase constituent Pam17 is specifically recruited by the receptor Tim50 to promote the transport of hypersensitive precursors into the mitochondrial matrix.
Title: Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation.
crystal structure of Tim50(164-361) at 2.67 A resolution
Title: The structure of Tim50(164-361) suggests the mechanism by which Tim50 receives mitochondrial presequences.
The C-terminal domain of Tim50 contributes to complex formation with Tim23.
Title: Molecular basis of the dynamic structure of the TIM23 complex in the mitochondrial intermembrane space.
The presequence binding domain can bind to the core domain of Tim50 through the presequence binding region.
Title: NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.
The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.
Title: Signal recognition initiates reorganization of the presequence translocase during protein import.
The beta-hairpin is crucial for the interaction of Tim50 with Tim23, suggesting a cooperative function of these two essential TIM proteins in preprotein import.
Title: Structural basis for the function of Tim50 in the mitochondrial presequence translocase.
Tim50 and its association with TIM23 has a role in the transfer of protein precursors between the inner and outer mitochondrial membrane.
Title: Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria.
Interaction of Tim23 with Tim50 Is essential for protein translocation by the mitochondrial TIM23 complex.
Title: Interaction of Tim23 with Tim50 Is essential for protein translocation by the mitochondrial TIM23 complex.
the intermembrane space domain of Tim50 induced the Tim23 channel to close
Title: Tim50 maintains the permeability barrier of the mitochondrial inner membrane.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mitochondrion targeting sequence binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to protein transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in protein import into mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of mitochondrial membrane permeability	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of TIM23 mitochondrial import inner membrane translocase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TIM23 mitochondrial import inner membrane translocase complex	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein translocase subunit TIM50

NP_015262.1

Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

429939..431369

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183877.1 → NP_015262.1 TPA: protein translocase subunit TIM50 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015262.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3V2, Q02776

UniProtKB/TrEMBL

A6ZWI6, B3LL06, B5VT99, C7GTZ4, C8ZIW7, G2WPB7, N1NW46

Conserved Domains (1) summary

COG5190
Location:1 → 354

FCP1; TFIIF-interacting CTD phosphatase, includes NLI-interacting factor [Transcription]