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    AI4 intron-encoded DNA endonuclease aI4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854596, updated on 9-Dec-2024

    Summary

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    Official Symbol
    AI4
    Official Full Name
    intron-encoded DNA endonuclease aI4
    Primary source
    SGD:S000007264
    Locus tag
    Q0065
    See related
    AllianceGenome:SGD:S000007264; FungiDB:Q0065; VEuPathDB:Q0065
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C, substrain: FY1679)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables endonuclease activity. Involved in RNA splicing and intron homing. Predicted to be located in mitochondrion. Predicted to be part of respiratory chain complex IV. Orthologous to human MT-CO1 (mitochondrially encoded cytochrome c oxidase I). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See AI4 in Genome Data Viewer
    Sequence:
    Chromosome: MT; NC_001224.1 (13818..21935)

    Chromosome MT - NC_001224.1Genomic Context describing neighboring genes Neighboring gene 15S ribosomal RNA Neighboring gene tRNA-Trp Neighboring gene intron-encoded DNA endonuclease aI5 alpha Neighboring gene cytochrome c oxidase subunit 1 Neighboring gene intron-encoded reverse transcriptase aI1 Neighboring gene intron-encoded reverse transcriptase aI2 Neighboring gene intron-encoded DNA endonuclease aI3 Neighboring gene intron-encoded DNA endonuclease aI5 beta Neighboring gene F1F0 ATP synthase subunit 8

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001224.1

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Maturase and endonuclease functions depend on separate conserved domains of the bifunctional protein encoded by the group I intron aI4 alpha of yeast mitochondrial DNA. Henke RM, et al. EMBO J, 1995 Oct 16. PMID 7588637, Free PMC Article
    2. Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Dujardin G, et al. Nature, 1982 Aug 12. PMID 6285204
    3. In vivo and in vitro analyses of an intron-encoded DNA endonuclease from yeast mitochondria. Recognition site by site-directed mutagenesis. Sargueil B, et al. Nucleic Acids Res, 1990 Oct 11. PMID 2216759, Free PMC Article
    4. Purification of a site-specific endonuclease, I-Sce II, encoded by intron 4 alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae. Wernette CM, et al. J Biol Chem, 1990 Nov 5. PMID 2172241
    5. Dissecting and analyzing the secondary structure domains of group I introns through the use of chimeric intron constructs. Tanner NK, et al. J Mol Biol, 1995 Oct 6. PMID 7563076

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    contributes_to cytochrome-c oxidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cytochrome-c oxidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA splicing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in aerobic respiration IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in aerobic respiration IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intron homing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intron homing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in respiratory electron transport chain IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion TAS
    Traceable Author Statement
    more info
    		 PubMed 
    part_of respiratory chain complex IV IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    intron-encoded DNA endonuclease aI4
    NP_009307.2
    • Endonuclease I-SceII; encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001224.1 Reference assembly

      Range
      13818..21935
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_009307.2 intron-encoded DNA endonuclease aI4 (mitochondrion) [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009307.2

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      A0A0A7P354, P03878, Q02339, Q35798, Q9ZZX2
      Conserved Domains (2) summary
      COG0843
      Location:1 → 555
      CyoB; Heme/copper-type cytochrome/quinol oxidase, subunit 1 [Energy production and conversion]
      pfam00115
      Location:13 → 240
      COX1; Cytochrome C and Quinol oxidase polypeptide I
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P03878.3

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