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    murF D-alanyl-D-alanine-adding enzyme [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944813, updated on 3-Dec-2024

    Summary

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    Official Symbol
    murF
    Official Full Name
    D-alanyl-D-alanine-adding enzyme
    Primary source
    ECOCYC:EG10622
    Locus tag
    b0086
    See related
    ASAP:ABE-0000313
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0087; mra
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    Genomic context

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    Sequence:
    NC_000913.3 (94650..96008)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 16S rRNA m(4)C1402 methyltransferase Neighboring gene cell division protein FtsL Neighboring gene peptidoglycan DD-transpeptidase FtsI Neighboring gene ncRNA Neighboring gene UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Neighboring gene phospho-N-acetylmuramoyl-pentapeptide-transferase Neighboring gene UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase Neighboring gene peptidoglycan glycosyltransferase FtsW Neighboring gene N-acetylglucosaminyl transferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli. Duncan K, et al. Biochemistry, 1990 Mar 6. PMID 2186811
    2. MurF inhibitors with antibacterial activity: effect on muropeptide levels. Baum EZ, et al. Antimicrob Agents Chemother, 2009 Aug. PMID 19470511, Free PMC Article
    3. Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli. Parquet C, et al. Nucleic Acids Res, 1989 Jul 11. PMID 2668880, Free PMC Article
    4. A MurF inhibitor that disrupts cell wall biosynthesis in Escherichia coli. Baum EZ, et al. Antimicrob Agents Chemother, 2007 Dec. PMID 17908943, Free PMC Article
    5. Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion. Anderson MS, et al. Biochemistry, 1996 Dec 17. PMID 8973200

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on complete protein
      Title: Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acid-amino acid ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in peptidoglycan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    D-alanyl-D-alanine-adding enzyme
    NP_414628.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      94650..96008
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414628.1 D-alanyl-D-alanine-adding enzyme [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414628.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A776LN25, A0A8T6BIA4, A0A8T6PMM5
      Conserved Domains (1) summary
      PRK10773
      Location:1 → 452
      murF; UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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