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    rusA crossover junction endodeoxyribonuclease RusA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945174, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rusA
    Official Full Name
    crossover junction endodeoxyribonuclease RusA
    Primary source
    ECOCYC:G6306
    Locus tag
    b0550
    See related
    ASAP:ABE-0001880
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0541; rus; ybcP
    Summary
    The RusA protein is an endonuclease that functions as a Holliday junction resolvase . [More information is available at EcoCyc: G6306].
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    Genomic context

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    Sequence:
    NC_000913.3 (573371..573733)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein YlcH Neighboring gene DNA base-flipping protein Neighboring gene NinE family prophage protein Neighboring gene putative nuclease YbcO Neighboring gene uncharacterized protein YlcG Neighboring gene prophage antitermination protein Q homolog QuuD Neighboring gene pseudo Neighboring gene IS5 family transposase and trans-activator

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Using transposon mutagenesis to find an alternative resolvase in an Escherichia coli cells lacking RuvABC. Jaktaji RP. Pak J Biol Sci, 2009 Mar 15. PMID 19580006
    2. Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli. Chan SN, et al. J Biol Chem, 1997 Jun 6. PMID 9169457
    3. Crystallization of RusA Holliday junction resolvase from Escherichia coli. Muranova TA, et al. Acta Crystallogr D Biol Crystallogr, 2003 Dec. PMID 14646089
    4. The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold. Rafferty JB, et al. Structure, 2003 Dec. PMID 14656440
    5. Resolution of Holliday intermediates in recombination and DNA repair: indirect suppression of ruvA, ruvB, and ruvC mutations. Mandal TN, et al. J Bacteriol, 1993 Jul. PMID 8331065, Free PMC Article

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on complete protein
      Title: Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions.
    2. The structure of a RusA-Holliday junction complex suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.
      Title: RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables crossover junction DNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables crossover junction DNA endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables four-way junction DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    crossover junction endodeoxyribonuclease RusA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      573371..573733
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415082.1 crossover junction endodeoxyribonuclease RusA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415082.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FXG3
      Conserved Domains (1) summary
      PRK09786
      Location:1 → 120
      PRK09786; endodeoxyribonuclease RUS; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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