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    ndh NADH:quinone oxidoreductase II [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946792, updated on 17-Dec-2024

    Summary

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    Official Symbol
    ndh
    Official Full Name
    NADH:quinone oxidoreductase II
    Primary source
    ECOCYC:EG10649
    Locus tag
    b1109
    See related
    ASAP:ABE-0003746
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1095
    Summary
    NADH:ubiquinone oxidoreductase II (NDH-2) is a type IIA NADH dehydrogenase that catalyzes the transfer of electrons from NADH to the quinone pool in the cytoplasmic membrane. [More information is available at EcoCyc: EG10649].
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    Genomic context

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    Sequence:
    NC_000913.3 (1166085..1167389)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene beta-N-acetylglucosaminidase Neighboring gene PF05728 family protein YcfP Neighboring gene PF05433 family protein YcfJ Neighboring gene DNA-binding transcriptional repressor ComR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. In vitro synthesis of the respiratory NADH dehydrogenase of Escherichia coli. Role of UUG as initiation codon. Poulis MI, et al. Biochemistry, 1981 Jul 7. PMID 7025892
    2. Evidence for Cu(I)-thiolate ligation and prediction of a putative copper-binding site in the Escherichia coli NADH dehydrogenase-2. Rapisarda VA, et al. Arch Biochem Biophys, 2002 Sep 1. PMID 12176061
    3. Quenching of bathocuproine disulfonate fluorescence by Cu(I) as a basis for copper quantification. Rapisarda VA, et al. Anal Biochem, 2002 Aug 1. PMID 12137786
    4. Purification of the 45 kDa, membrane bound NADH dehydrogenase of Escherichia coli (NDH-2) and analysis of its interaction with ubiquinone analogues. Björklöf K, et al. FEBS Lett, 2000 Feb 4. PMID 10664466
    5. Characterization of an NADH-linked cupric reductase activity from the Escherichia coli respiratory chain. Rapisarda VA, et al. Arch Biochem Biophys, 1999 Oct 15. PMID 10510271

    See all (54) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Because of the specific stabilization of substrate complexes of EcNDH-2 immobilized on electrodes, it was possible to demonstrate the presence of two distinct substrate binding sites for NADH and the quinone and to identify a bound semiprotonated quinol as a catalytic intermediate.
      Title: Substrate-Protein Interactions of Type II NADH:Quinone Oxidoreductase from Escherichia coli.
    2. Improved poly(3-hydroxybutyrate) production in Escherichia coli by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains
      Title: Improved poly(3-hydroxybutyrate) production in Escherichia coli by inactivation of cytochrome bd-II oxidase or/and NDH-II dehydrogenase in low efficient respiratory chains.
    3. The paper reports that the maintenance of NDH-2 activity and ndh expression in the stationary phase when cells were grown in media containing at least 37 mM phosphate.
      Title: A critical phosphate concentration in the stationary phase maintains ndh gene expression and aerobic respiratory chain activity in Escherichia coli.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables NADH dehydrogenase (ubiquinone) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables NADH dehydrogenase (ubiquinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NADH:ubiquinone reductase (non-electrogenic) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cupric reductase (NADH) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables flavin adenine dinucleotide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within aerobic electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within aerobic respiration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within anaerobic respiration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within copper ion homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of NADH dehydrogenase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    NADH:quinone oxidoreductase II
    NP_415627.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1166085..1167389
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415627.1 NADH:quinone oxidoreductase II [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415627.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3IAR3, A0A1X3JJI2, A0A829LAY4, A0AAN3SDJ5
      Conserved Domains (1) summary
      COG1252
      Location:6 → 423
      Ndh; NADH dehydrogenase, FAD-containing subunit [Energy production and conversion]
    Nucleotide Protein
    Heading Accession and Version

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