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    ispG (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946991, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ispG
    Official Full Name
    (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
    Primary source
    ECOCYC:EG10370
    Locus tag
    b2515
    See related
    ASAP:ABE-0008281
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2511; gcpE
    Summary
    The IspG substrate is 2C-methyl-d-erythritol 2,4-cyclodiphosphate. [More information is available at EcoGene: EG10370]. 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) catalyzes the sixth step in the methylerythritol phosphate pathway. [More information is available at EcoCyc: EG10370].
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    Genomic context

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    Sequence:
    NC_000913.3 (2640686..2641804, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene histidine--tRNA ligase Neighboring gene ncRNA Neighboring gene cytoskeleton protein RodZ Neighboring gene 23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Biosynthesis of isoprenoids. purification and properties of IspG protein from Escherichia coli. Zepeck F, et al. J Org Chem, 2005 Nov 11. PMID 16268586
    2. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Seemann M, et al. Angew Chem Int Ed Engl, 2002 Nov 15. PMID 12434382
    3. IspG converts an epoxide substrate analogue to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate: implications for IspG catalysis in isoprenoid biosynthesis. Nyland RL 2nd, et al. J Am Chem Soc, 2009 Dec 16. PMID 19919056, Free PMC Article
    4. IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster. Xiao Y, et al. Biochemistry, 2009 Nov 10. PMID 19821611
    5. Cutting edge: human gamma delta T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis. Altincicek B, et al. J Immunol, 2001 Mar 15. PMID 11238603

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production.
      Title: Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli.
    2. analysis of the organometallic mechanism of action and inhibition of the 4Fe-4S isoprenoid biosynthesis protein GcpE (IspG)
      Title: Organometallic mechanism of action and inhibition of the 4Fe-4S isoprenoid biosynthesis protein GcpE (IspG).
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase activity (flavodoxin) IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in isoprenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in terpenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
    NP_417010.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2640686..2641804 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417010.1 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417010.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7T2J2W8
      Conserved Domains (1) summary
      PRK00366
      Location:1 → 359
      ispG; flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
    Nucleotide Protein
    Heading Accession and Version

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