U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    rplL 50S ribosomal subunit protein L12 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948489, updated on 17-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    rplL
    Official Full Name
    50S ribosomal subunit protein L12
    Primary source
    ECOCYC:EG10873
    Locus tag
    b3986
    See related
    ASAP:ABE-0013038
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3977
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4180560..4180925)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L1 Neighboring gene 50S ribosomal subunit protein L10 Neighboring gene RNA polymerase subunit beta Neighboring gene RNA polymerase subunit beta'

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A point mutation in ribosomal protein L7/L12 reduces its ability to form a compact dimer structure and to assemble into the GTPase center. Nomura T, et al. Biochemistry, 2003 Apr 29. PMID 12705832
    2. Ribosomal proteins L7/L12 of Escherichia coli. Localization and possible molecular mechanism in translation. Möller W, et al. J Mol Biol, 1983 Feb 5. PMID 6133002
    3. Letter: Inhibition of protein L7-L12 binding to 50 S ribosomal cores by antibodies specific for proteins L6, L10 and L18. Stöffler G, et al. J Mol Biol, 1974 Jun 15. PMID 4852827
    4. NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit. Zhao Q, et al. Nucleic Acids Res, 2005. PMID 15939932, Free PMC Article
    5. Chemistry and biology of E. coli ribosomal protein L12. Brot N, et al. Mol Cell Biochem, 1981 Apr 13. PMID 7017380

    See all (88) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Alterations in the ribosomal protein bL12 of E. coli affecting the initiation, elongation and termination of protein synthesis.
      Title: Alterations in the ribosomal protein bL12 of E. coli affecting the initiation, elongation and termination of protein synthesis.
    2. MD simulations of IF2 docked onto the L12-CTD pinpointed the exact interacting partners-all of which were positively charged on L12 and negatively charged on IF2, connected by salt bridges.
      Title: Complementary charge-based interaction between the ribosomal-stalk protein L7/12 and IF2 is the key to rapid subunit association.
    3. Upon removal of L7/L12 from ribosomes, the GTPase activities of EF-G, RF3, and IF2 decreased to basal levels, while the activity of LepA decreased marginally. Upon reconstitution of ribosomes with recombinant L12, the GTPase activities of all GTPases returned to full activity.
      Title: Ribosomal protein L7/L12 is required for GTPase translation factors EF-G, RF3, and IF2 to bind in their GTP state to 70S ribosomes.
    4. Data show that acetylation of L12 increases interactions in the Escherichia coli ribosomal stalk complex.
      Title: Acetylation of L12 increases interactions in the Escherichia coli ribosomal stalk complex.
    5. N-terminus verified by Edman degradation on mature peptide
      Title: A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification.
    6. control of Pi release by interactions between EF-G and L7/12 appears to be important for maintaining the conformational coupling between EF-G and the ribosome for translocation and for timing the dissociation of the factor from the ribosome
      Title: Control of phosphate release from elongation factor G by ribosomal protein L7/12.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    contributes_to GTPase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables mRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic large ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of large ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    50S ribosomal subunit protein L12

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4180560..4180925
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418413.1 50S ribosomal subunit protein L12 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418413.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAV3H2V4
      Conserved Domains (1) summary
      PRK00157
      Location:1 → 121
      rplL; 50S ribosomal protein L7/L12; Reviewed
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.