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    uvrB UvrABC excision nuclease subunit B [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945385, updated on 3-Dec-2024

    Summary

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    Official Symbol
    uvrB
    Official Full Name
    UvrABC excision nuclease subunit B
    Primary source
    ECOCYC:EG11062
    Locus tag
    b0779
    See related
    ASAP:ABE-0002658
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dar-1; dar-6; ECK0768
    Summary
    [More information is available at EcoCyc: EG11062].
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    Genomic context

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    Sequence:
    NC_000913.3 (813526..815547)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene biotin synthase Neighboring gene 8-amino-7-oxononanoate synthase Neighboring gene malonyl-acyl carrier protein methyltransferase Neighboring gene dethiobiotin synthetase Neighboring gene putative transferase YbhK Neighboring gene GTP 3',8'-cyclase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Functions of base flipping in E. coli nucleotide excision repair. Malta E, et al. DNA Repair (Amst), 2008 Oct 1. PMID 18638572
    2. Introduction of a tryptophan reporter group into the ATP binding motif of the Escherichia coli UvrB protein for the study of nucleotide binding and conformational dynamics. Hildebrand EL, et al. J Biol Chem, 1998 Apr 3. PMID 9525874
    3. Accessibility of epitopes on UvrB protein in intermediates generated during incision of UV-irradiated DNA by the Escherichia coli Uvr(A)BC endonuclease. Kovalsky OI, et al. J Biol Chem, 1998 Aug 14. PMID 9694852
    4. Detection of DNA damage by Escherichia coli UvrB-binding competition assay is limited by the stability of the UvrB-DNA complex. Routledge MN, et al. Carcinogenesis, 1997 Jul. PMID 9230288
    5. Thermodynamic characterization of the interaction of mutant UvrB protein with damaged DNA. Ma H, et al. Biochemistry, 2004 Apr 13. PMID 15065864, Free PMC Article

    See all (103) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
      Title: Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
    2. Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases bacterial survival.
      Title: Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival.
    3. ATPase domain, ATP binding domain and/or the DNA binding domain of the UvrB protein are required for DNA repair.
      Title: Evidence for involvement of UvrB in elicitation of 'SIR' phenotype by rpoB87-gyrA87 mutations in lexA3 mutant of Escherichia coli.
    4. study visualized how UvrA and UvrB interacted with DNA individually or together by single- molecule imaging of quantum-dot-labeled proteins
      Title: Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins.
    5. found that the N-terminal domain and the unstructured region at the C-terminus of UvrD interact with UvrB.
      Title: The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair.
    6. Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB.
      Title: Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB.
    7. N-terminus verified by Edman degradation on mature peptide
      Title: Sequences of the E. coli uvrB gene and protein.
    8. In solution, two UvrB subunits bind to UvrA, most likely as part of a UvrA2B2 complex. The UvrA-induced dimerization of the C-terminal domain 4 regions of UvrB serves to shield these domains from premature UvrC binding.
      Title: Dynamics of the UvrABC nucleotide excision repair proteins analyzed by fluorescence resonance energy transfer.
    9. transcription-repair ATP-dependent coupling factor-N reveals a fold that resembles the three N-terminal domains of the repair enzyme UvrB
      Title: Structural basis for transcription-coupled repair: the N terminus of Mfd resembles UvrB with degenerate ATPase motifs.
    10. we examined the interaction of UvrABC with a DNA substrate containing a single G[8,5-Me]T cross-link and compared it with T[6,4]T (the 6-4 pyrimidine-pyrimidone photoproduct) and the C8 guanine adduct of N-acetyl-2-aminofluorene (AAF)
      Title: Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables excinuclease ABC activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables nuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in SOS response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleotide-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nucleotide-excision repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleotide-excision repair NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, DNA damage recognition IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleotide-excision repair, DNA damage recognition IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, preincision complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of excinuclease repair complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of excinuclease repair complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of excinuclease repair complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of excinuclease repair complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    UvrABC excision nuclease subunit B

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      813526..815547
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415300.1 UvrABC excision nuclease subunit B [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415300.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7FF61
      Conserved Domains (1) summary
      PRK05298
      Location:1 → 673
      PRK05298; excinuclease ABC subunit UvrB
    Nucleotide Protein
    Heading Accession and Version

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