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    proP osmolyte:H(+) symporter ProP [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948626, updated on 3-Dec-2024

    Summary

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    Official Symbol
    proP
    Official Full Name
    osmolyte:H(+) symporter ProP
    Primary source
    ECOCYC:EG11612
    Locus tag
    b4111
    See related
    ASAP:ABE-0013462
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4104
    Summary
    Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced-expression subset. [More information is available at EcoGene: EG11612]. ProP is an osmoprotectant/proton symporter capable of transporting proline and glycine betaine, and mediates the uptake of osmoprotectants to adapt to increases in osmotic pressure. [More information is available at EcoCyc: EG11612].
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    Genomic context

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    Sequence:
    NC_000913.3 (4330502..4332004)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene zinc ribbon domain-containing protein YjdM Neighboring gene regulator of diguanylate cyclase RdcA Neighboring gene regulator of diguanylate cyclase RdcB Neighboring gene small regulatory membrane protein PmrR Neighboring gene sensor histidine kinase BasS Neighboring gene DNA-binding transcriptional dual regulator BasR Neighboring gene phosphoethanolamine transferase EptA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Nanomolar levels of dimethylsulfoniopropionate, dimethylsulfonioacetate, and glycine betaine are sufficient to confer osmoprotection to Escherichia coli. Cosquer A, et al. Appl Environ Microbiol, 1999 Aug. PMID 10427011, Free PMC Article
    2. Identification and mapping of a second proline permease Salmonella typhimurium. Menzel R, et al. J Bacteriol, 1980 Mar. PMID 6245058, Free PMC Article
    3. Detection of alpha-helical coiled-coil dimer formation by spin-labeled synthetic peptides: a model parallel coiled-coil peptide and the antiparallel coiled coil formed by a replica of the ProP C-terminus. Hillar A, et al. Biochemistry, 2003 Dec 30. PMID 14690427
    4. Solution structure of the C-terminal antiparallel coiled-coil domain from Escherichia coli osmosensor ProP. Zoetewey DL, et al. J Mol Biol, 2003 Dec 12. PMID 14643666
    5. Osmosensing and osmoregulatory compatible solute accumulation by bacteria. Wood JM, et al. Comp Biochem Physiol A Mol Integr Physiol, 2001 Oct. PMID 11913457

    See all (79) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.
      Title: Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.
    2. ProP adopts a highly alpha-helical fold similar to that of LacY, and both transmembrane folds exhibit unusually high temperature-sensitive solvent accessibility
      Title: Structural characterization of the osmosensor ProP.
    3. Periplasmic loops of osmosensory transporter ProP in Escherichia coli are sensitive to osmolality
      Title: Periplasmic loops of osmosensory transporter ProP in Escherichia coli are sensitive to osmolality.
    4. ProP switches among "off", "on", and "osmolality-sensitive" states as the membrane potential increases.
      Title: Roles of K+, H+, H2O, and DeltaPsi in solute transport mediated by major facilitator superfamily members ProP and LacY.
    5. molecular model predicts the presence of a proton pathway within the N-terminal six-helix bundle of ProP
      Title: A structural model for the osmosensor, transporter, and osmoregulator ProP of Escherichia coli.
    6. These studies define position of ProP TM XII within the membrane, further support predicted structure of ProP, reveal the dimerization interface, and show that the structure of transmembrane segment XII influences the osmolality at which ProP activates.
      Title: Structure and function of transmembrane segment XII in osmosensor and osmoprotectant transporter ProP of Escherichia coli.
    7. Association of proP with cardiolipin (and not phosphatidylglycerol) may be required for polar ProP localization.
      Title: Cardiolipin promotes polar localization of osmosensory transporter ProP in Escherichia coli.
    8. study elucidates rules governing RpoS selectivity in presence of a class II activator, provide insight into transcriptional activation by Fis from this position & clarify, why the proP promoter is activated during a short time when Fis & RpoS are present
      Title: The -35 sequence location and the Fis-sigma factor interface determine sigmas selectivity of the proP (P2) promoter in Escherichia coli.
    9. ProP osmotic activation is tuned by both its C-terminal coiled-coil and osmotically induced changes in phospholipid composition
      Title: The osmotic activation of transporter ProP is tuned by both its C-terminal coiled-coil and osmotically induced changes in phospholipid composition.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables proline:proton symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within amino acid transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular hyperosmotic response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cellular hyperosmotic salinity response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within glycine betaine transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in osmosensory signaling pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within proline import across plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proline import across plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within proline import across plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    osmolyte:H(+) symporter ProP

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4330502..4332004
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418535.1 osmolyte:H(+) symporter ProP [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418535.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MF91, A0A6N8N9K8
      Conserved Domains (1) summary
      PRK10642
      Location:10 → 499
      PRK10642; proline/glycine betaine transporter ProP
    Nucleotide Protein
    Heading Accession and Version

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